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Dual-Labeled BHQ® Probes


Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences.

Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact techsupport@biosearchtech.com with any questions regarding probe design.

NOTE:
Building Probes with an Internal Quencher
(Currently available with FAM only)
Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification.
Building Probes with Wobble/Degenerate Bases (What are Wobbles?)
To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc.

See also:
qPCR Assays | SNP Assays | BHQplus® Probes | Molecular Beacons | Scorpions® Primers | Dye Calibration Standards | Dyes from Biosearch Technologies
  Best DEAL:
  • $95 ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe delivered in 2 business days
Instrument CALIBRATION: Learn MORE:
Assay DESIGN:
  • Try RealTimeDesign™ Software, a FREE,  qPCR assay design program.  Design your Dual-Labeled BHQ probes and primers online today!
Access RealTimeDesign




Product Listings

Catalog # Item Name Price Size/Scale Note
DLO-RFB-5 ValuProbe™, 5' FAM/3' BHQ-1 $95.00 50 nmol Provides 10 nmol pure.
DLO-FB1-5 Dual-labeled Probe, 5' FAM/3' BHQ-1 $150.00 100 nmol Provides > 10 nmol pure.
DLO-FB1-2 Dual-labeled Probe, 5' FAM/3' BHQ-1 $250.00 200 nmol Provides > 25 nmol pure.
DLO-FB1-1 Dual-labeled Probe, 5' FAM/3' BHQ-1 $365.00 1 µmol Provides > 60 nmol pure.
DLO-F(T-B1)-2 Dual-labeled Probe, 5' FAM/Internal T-BHQ-1/3’ C3 $295.00 200 nmol Provides > 10 nmol pure.
DLO-F(T-B1)-1 Dual-labeled Probe, 5' FAM/Internal T-BHQ-1/3’ C3 $515.00 1 µmol Provides > 30 nmol pure.
DLO-FT-5 Dual-labeled Probe, 5' FAM/3' TAMRA $150.00 100 nmol Provides > 10 nmol pure.
DLO-FT-25 Dual-labeled Probe, 5' FAM/3' TAMRA $120.00 25 nmol Provides 5 nmol pure.
DLO-FT-2 Dual-labeled Probe, 5' FAM/3' TAMRA $250.00 200 nmol Provides > 25 nmol pure.
DLO-FT-1 Dual-labeled Probe, 5' FAM/3' TAMRA $365.00 1 µmol Provides > 60 nmol pure.
DLO-TEB1-5 Dual-labeled Probe, 5' TET/3' BHQ-1 $200.00 50 nmol Provides > 10 nmol pure.
DLO-TEB1-25 Dual-labeled Probe, 5' TET/3' BHQ-1 $120.00 25 nmol Provides 5 nmol pure.
DLO-TEB1-2 Dual-labeled Probe, 5' TET/3' BHQ-1 $290.00 200 nmol Provides > 25 nmol pure.
DLO-TEB1-1 Dual-labeled Probe, 5' TET/3' BHQ-1 $420.00 1 µmol Provides > 60 nmol pure.
DLO-TET-5 Dual-labeled Probe, 5' TET/3' TAMRA $200.00 50 nmol Provides > 10 nmol pure.
DLO-TET-25 Dual-labeled Probe, 5' TET/3' TAMRA $120.00 25 nmol Provides 5 nmol pure.
DLO-TET-2 Dual-labeled Probe, 5' TET/3' TAMRA $295.00 200 nmol Provides > 25 nmol pure.
DLO-TET-1 Dual-labeled Probe, 5' TET/3' TAMRA $420.00 1 µmol Provides > 60 nmol pure.
DLO-CGB1-5 Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1 $195.00 50 nmol Provides > 10 nmol pure.
DLO-CGB1-25 Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1 $120.00 25 nmol Provides 5 nmol pure.
DLO-CGB1-2 Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1 $275.00 200 nmol Provides > 25 nmol pure.
DLO-CGB1-1 Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1 $400.00 1 µmol Provides > 60 nmol pure.
DLO-JB1-2 Dual-labeled Probe, 5' JOE/3' BHQ-1 $450.00 200 nmol Provides > 10 nmol pure.
DLO-JB1-1 Dual-labeled Probe, 5' JOE/3' BHQ-1 $600.00 1 µmol Provides > 30 nmol pure.
DLO-HB1-5 Dual-labeled Probe, 5' HEX/3' BHQ-1 $205.00 50 nmol Provides > 10 nmol pure.
DLO-HB1-25 Dual-labeled Probe, 5' HEX/3' BHQ-1 $120.00 25 nmol Provides 5 nmol pure.
DLO-HB1-2 Dual-labeled Probe, 5' HEX/3' BHQ-1 $310.00 200 nmol Provides > 25 nmol pure.
DLO-HB1-1 Dual-labeled Probe, 5' HEX/3' BHQ-1 $450.00 1 µmol Provides > 60 nmol pure.
DLO-COB1-5 Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1 $195.00 50 nmol Provides > 10 nmol pure.
DLO-COB1-25 Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1 $120.00 25 nmol Provides 5 nmol pure.
DLO-COB1-2 Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1 $275.00 200 nmol Provides > 25 nmol pure.
DLO-COB1-1 Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1 $400.00 1 µmol Provides > 60 nmol pure.
DLO-Q5B2-5 Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2 $215.00 50 nmol Provides > 10 nmol pure.
DLO-Q5B2-25 Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2 $120.00 25 nmol Provides 5 nmol pure.
DLO-Q5B2-2 Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2 $315.00 200 nmol Provides > 25 nmol pure.
DLO-Q5B2-1 Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2 $450.00 1 µmol Provides > 60 nmol pure.
DLO-TB2-5 Dual-labeled Probe, 5' TAMRA/3' BHQ-2 $200.00 50 nmol Provides > 10 nmol pure.
DLO-TB2-25 Dual-labeled Probe, 5' TAMRA/3' BHQ-2 $120.00 25 nmol Provides 5 nmol pure
DLO-TB2-2 Dual-labeled Probe, 5' TAMRA/3' BHQ-2 $280.00 200 nmol Provides > 25 nmol pure.
DLO-TB2-1 Dual-labeled Probe, 5' TAMRA/3' BHQ-2 $400.00 1 µmol Provides > 60 nmol pure.
DLO-RB2-2 Dual-labeled Probe, 5' ROX/3' BHQ-2 $450.00 200 nmol Provides > 10 nmol pure.
DLO-RB2-1 Dual-labeled Probe, 5' ROX/3' BHQ-2 $600.00 1 µmol Provides > 30 nmol pure.
DLO-C590B2-5 Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2 $195.00 50 nmol Provides > 10 nmol pure.
DLO-C590B2-25 Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2 $120.00 25 nmol Provides 5 nmol pure.
DLO-C590B2-2 Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2 $270.00 200 nmol Provides > 25 nmol pure.
DLO-C590B2-1 Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2 $390.00 1 µmol Provides > 60 nmol pure.
DLO-CAB2-5 Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2 $215.00 50 nmol Provides > 10 nmol pure.
DLO-CAB2-25 Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2 $120.00 25 nmol Provides 5 nmol pure.
DLO-CAB2-2 Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2 $315.00 200 nmol Provides > 25 nmol pure.
DLO-CAB2-1 Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2 $475.00 1 µmol Provides > 60 nmol pure.
DLO-C635B2-5 Dual-labeled Probe, 5' CAL Fluor Red 635/3' BHQ-2 $255.00 50 nmol Provides > 3 nmol pure.
DLO-C635B2-2 Dual-labeled Probe, 5' CAL Fluor Red 635/3' BHQ-2 $375.00 200 nmol Provides > 10 nmol pure.
DLO-C635B2-1 Dual-labeled Probe, 5' CAL Fluor Red 635/3' BHQ-2 $550.00 1 µmol Provides > 40 nmol pure.
DLO-B2P6-2 Dual-labeled Probe, 5’ BHQ-2/3’ Pulsar 650 $450.00 200 nmol Provides > 10 nmol pure.
DLO-B2P6-1 Dual-labeled Probe, 5’ BHQ-2/3’ Pulsar 650 $660.00 1 µmol Provides > 40 nmol pure.
DLO-Q6B2-5 Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2 $230.00 50 nmol Provides 10 nmol pure.
DLO-Q6B2-25 Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2 $120.00 25 nmol Provides 5 nmol pure.
DLO-Q6B2-2 Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2 $315.00 200 nmol Provides > 20 nmol pure.
DLO-Q6B2-1 Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2 $475.00 1 µmol Provides > 40 nmol pure.
DLO-Q6B3-5 Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3 $230.00 50 nmol Provides 10 nmol pure.
DLO-Q6B3-25 Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3 $120.00 25 nmol Provides 5 nmol pure.
DLO-Q6B3-2 Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3 $315.00 200 nmol Provides > 20 nmol pure.
DLO-Q6B3-1 Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3 $475.00 1 µmol Provides > 40 nmol pure.
DLO-Q7B2-5 Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2 $230.00 50 nmol Provides > 3 nmol pure.
DLO-Q7B2-2 Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2 $315.00 200 nmol Provides > 10 nmol pure.
DLO-Q7B2-1 Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2 $475.00 1 µmol Provides > 40 nmol pure.
DLO-Q7B3-5 Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3 $230.00 50 nmol Provides > 3 nmol pure.
DLO-Q7B3-2 Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3 $315.00 200 nmol Provides > 10 nmol pure.
DLO-Q7B3-1 Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3 $475.00 1 µmol Provides > 40 nmol pure.
P2-5 Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each) $14.00 50 nmol Provides 20-40 nmol.
P2-2 Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each) $25.00 200 nmol Provides 80-160 nmol.
P2-1 Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each) $40.00 1 µmol Provides 400-800 nmol.
P2C-5 Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each) $30.00 50 nmol Provides 11.25-22.5 nmol pure.
P2C-2 Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each) $35.00 200 nmol Provides 45-90 nmol pure.
P2C-1 Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each) $50.00 1 µmol Provides 225-450 nmol pure.



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