Dual-Labeled BHQ® Probes
| Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus™ probe instead, or else contact techsupport@biosearchtech.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. See also: BHQplus™ Probes | Molecular Beacons | Scorpions® Primers | Dye Calibration Standards | Dyes from Biosearch Technologies | Best DEAL:
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Product Listings
| Catalog # | Item Name | Price | Size/Scale | Note |
|---|---|---|---|---|
| DLO-RFB-5 | ValuProbe™, 5' FAM/3' BHQ-1 | $95.00 | 50 nmol | Provides 10 nmol pure. |
| DLO-FB1-5 | Dual-labeled Probe, 5' FAM/3' BHQ-1 | $150.00 | 100 nmol | Provides > 10 nmol pure. |
| DLO-FB1-2 | Dual-labeled Probe, 5' FAM/3' BHQ-1 | $250.00 | 200 nmol | Provides > 25 nmol pure. |
| DLO-FB1-1 | Dual-labeled Probe, 5' FAM/3' BHQ-1 | $365.00 | 1 µmol | Provides > 60 nmol pure. |
| DLO-F(T-B1)-2 | Dual-labeled Probe, 5' FAM/Internal T-BHQ-1/3’ C3 | $400.00 | 200 nmol | Provides > 10 nmol pure. |
| DLO-F(T-B1)-1 | Dual-labeled Probe, 5' FAM/Internal T-BHQ-1/3’ C3 | $515.00 | 1 µmol | Provides > 30 nmol pure. |
| DLO-FT-5 | Dual-labeled Probe, 5' FAM/3' TAMRA | $150.00 | 100 nmol | Provides > 10 nmol pure. |
| DLO-FT-25 | Dual-labeled Probe, 5' FAM/3' TAMRA | $120.00 | 25 nmol | Provides 5 nmol pure. |
| DLO-FT-2 | Dual-labeled Probe, 5' FAM/3' TAMRA | $250.00 | 200 nmol | Provides > 25 nmol pure. |
| DLO-FT-1 | Dual-labeled Probe, 5' FAM/3' TAMRA | $365.00 | 1 µmol | Provides > 60 nmol pure. |
| DLO-TEB1-5 | Dual-labeled Probe, 5' TET/3' BHQ-1 | $200.00 | 50 nmol | Provides > 10 nmol pure. |
| DLO-TEB1-25 | Dual-labeled Probe, 5' TET/3' BHQ-1 | $120.00 | 25 nmol | Provides 5 nmol pure. |
| DLO-TEB1-2 | Dual-labeled Probe, 5' TET/3' BHQ-1 | $290.00 | 200 nmol | Provides > 25 nmol pure. |
| DLO-TEB1-1 | Dual-labeled Probe, 5' TET/3' BHQ-1 | $420.00 | 1 µmol | Provides > 60 nmol pure. |
| DLO-TET-5 | Dual-labeled Probe, 5' TET/3' TAMRA | $200.00 | 50 nmol | Provides > 10 nmol pure. |
| DLO-TET-25 | Dual-labeled Probe, 5' TET/3' TAMRA | $120.00 | 25 nmol | Provides 5 nmol pure. |
| DLO-TET-2 | Dual-labeled Probe, 5' TET/3' TAMRA | $295.00 | 200 nmol | Provides > 25 nmol pure. |
| DLO-TET-1 | Dual-labeled Probe, 5' TET/3' TAMRA | $420.00 | 1 µmol | Provides > 60 nmol pure. |
| DLO-CGB1-5 | Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1 | $195.00 | 50 nmol | Provides > 10 nmol pure. |
| DLO-CGB1-25 | Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1 | $120.00 | 25 nmol | Provides 5 nmol pure. |
| DLO-CGB1-2 | Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1 | $275.00 | 200 nmol | Provides > 25 nmol pure. |
| DLO-CGB1-1 | Dual-labeled Probe, 5' CAL Fluor Gold 540/3' BHQ-1 | $400.00 | 1 µmol | Provides > 60 nmol pure. |
| DLO-JB1-2 | Dual-labeled Probe, 5' JOE/3' BHQ-1 | $450.00 | 200 nmol | Provides > 10 nmol pure. |
| DLO-JB1-1 | Dual-labeled Probe, 5' JOE/3' BHQ-1 | $600.00 | 1 µmol | Provides > 30 nmol pure. |
| DLO-HB1-5 | Dual-labeled Probe, 5' HEX/3' BHQ-1 | $205.00 | 50 nmol | Provides > 10 nmol pure. |
| DLO-HB1-25 | Dual-labeled Probe, 5' HEX/3' BHQ-1 | $120.00 | 25 nmol | Provides 5 nmol pure. |
| DLO-HB1-2 | Dual-labeled Probe, 5' HEX/3' BHQ-1 | $310.00 | 200 nmol | Provides > 25 nmol pure. |
| DLO-HB1-1 | Dual-labeled Probe, 5' HEX/3' BHQ-1 | $450.00 | 1 µmol | Provides > 60 nmol pure. |
| DLO-COB1-5 | Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1 | $195.00 | 50 nmol | Provides > 10 nmol pure. |
| DLO-COB1-25 | Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1 | $120.00 | 25 nmol | Provides 5 nmol pure. |
| DLO-COB1-2 | Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1 | $275.00 | 200 nmol | Provides > 25 nmol pure. |
| DLO-COB1-1 | Dual-labeled Probe, 5' CAL Fluor Orange 560/3' BHQ-1 | $400.00 | 1 µmol | Provides > 60 nmol pure. |
| DLO-Q5B2-5 | Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2 | $215.00 | 50 nmol | Provides > 10 nmol pure. |
| DLO-Q5B2-25 | Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2 | $120.00 | 25 nmol | Provides 5 nmol pure. |
| DLO-Q5B2-2 | Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2 | $315.00 | 200 nmol | Provides > 25 nmol pure. |
| DLO-Q5B2-1 | Dual-labeled Probe, 5' Quasar 570/3’ BHQ-2 | $450.00 | 1 µmol | Provides > 60 nmol pure. |
| DLO-TB2-5 | Dual-labeled Probe, 5' TAMRA/3' BHQ-2 | $200.00 | 50 nmol | Provides > 10 nmol pure. |
| DLO-TB2-25 | Dual-labeled Probe, 5' TAMRA/3' BHQ-2 | $120.00 | 25 nmol | Provides 5 nmol pure |
| DLO-TB2-2 | Dual-labeled Probe, 5' TAMRA/3' BHQ-2 | $280.00 | 200 nmol | Provides > 25 nmol pure. |
| DLO-TB2-1 | Dual-labeled Probe, 5' TAMRA/3' BHQ-2 | $400.00 | 1 µmol | Provides > 60 nmol pure. |
| DLO-RB2-2 | Dual-labeled Probe, 5' ROX/3' BHQ-2 | $450.00 | 200 nmol | Provides > 10 nmol pure. |
| DLO-RB2-1 | Dual-labeled Probe, 5' ROX/3' BHQ-2 | $600.00 | 1 µmol | Provides > 30 nmol pure. |
| DLO-C590B2-5 | Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2 | $195.00 | 50 nmol | Provides > 10 nmol pure. |
| DLO-C590B2-25 | Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2 | $120.00 | 25 nmol | Provides 5 nmol pure. |
| DLO-C590B2-2 | Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2 | $270.00 | 200 nmol | Provides > 25 nmol pure. |
| DLO-C590B2-1 | Dual-labeled Probe, 5' CAL Fluor Red 590/3' BHQ-2 | $390.00 | 1 µmol | Provides > 60 nmol pure. |
| DLO-CAB2-5 | Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2 | $215.00 | 50 nmol | Provides > 10 nmol pure. |
| DLO-CAB2-25 | Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2 | $120.00 | 25 nmol | Provides 5 nmol pure. |
| DLO-CAB2-2 | Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2 | $315.00 | 200 nmol | Provides > 25 nmol pure. |
| DLO-CAB2-1 | Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ-2 | $475.00 | 1 µmol | Provides > 60 nmol pure. |
| DLO-C635B2-5 | Dual-labeled Probe, 5' CAL Fluor Red 635/3' BHQ-2 | $255.00 | 50 nmol | Provides > 3 nmol pure. |
| DLO-C635B2-2 | Dual-labeled Probe, 5' CAL Fluor Red 635/3' BHQ-2 | $375.00 | 200 nmol | Provides > 10 nmol pure. |
| DLO-C635B2-1 | Dual-labeled Probe, 5' CAL Fluor Red 635/3' BHQ-2 | $550.00 | 1 µmol | Provides > 40 nmol pure. |
| DLO-B2P6-2 | Dual-labeled Probe, 5’ BHQ-2/3’ Pulsar 650 | $450.00 | 200 nmol | Provides > 10 nmol pure. |
| DLO-B2P6-1 | Dual-labeled Probe, 5’ BHQ-2/3’ Pulsar 650 | $660.00 | 1 µmol | Provides > 40 nmol pure. |
| DLO-Q6B2-5 | Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2 | $230.00 | 50 nmol | Provides 10 nmol pure. |
| DLO-Q6B2-25 | Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2 | $120.00 | 25 nmol | Provides 5 nmol pure. |
| DLO-Q6B2-2 | Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2 | $315.00 | 200 nmol | Provides > 20 nmol pure. |
| DLO-Q6B2-1 | Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2 | $475.00 | 1 µmol | Provides > 40 nmol pure. |
| DLO-Q6B3-5 | Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3 | $230.00 | 50 nmol | Provides 10 nmol pure. |
| DLO-Q6B3-25 | Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3 | $120.00 | 25 nmol | Provides 5 nmol pure. |
| DLO-Q6B3-2 | Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3 | $315.00 | 200 nmol | Provides > 20 nmol pure. |
| DLO-Q6B3-1 | Dual-labeled Probe, 5' Quasar 670/3’ BHQ-3 | $475.00 | 1 µmol | Provides > 40 nmol pure. |
| DLO-Q7B2-5 | Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2 | $230.00 | 50 nmol | Provides > 3 nmol pure. |
| DLO-Q7B2-2 | Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2 | $315.00 | 200 nmol | Provides > 10 nmol pure. |
| DLO-Q7B2-1 | Dual-labeled Probe, 5' Quasar 705/3’ BHQ-2 | $475.00 | 1 µmol | Provides > 40 nmol pure. |
| DLO-Q7B3-5 | Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3 | $230.00 | 50 nmol | Provides > 3 nmol pure. |
| DLO-Q7B3-2 | Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3 | $315.00 | 200 nmol | Provides > 10 nmol pure. |
| DLO-Q7B3-1 | Dual-labeled Probe, 5' Quasar 705/3’ BHQ-3 | $475.00 | 1 µmol | Provides > 40 nmol pure. |
| P2-5 | Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each) | $14.00 | 50 nmol | Provides 20-40 nmol. |
| P2-2 | Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each) | $25.00 | 200 nmol | Provides 80-160 nmol. |
| P2-1 | Primer Pair, (with probe purchase) Salt-Free (up to 30 bases each) | $40.00 | 1 µmol | Provides 400-800 nmol. |
| P2C-5 | Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each) | $30.00 | 50 nmol | Provides 11.25-22.5 nmol pure. |
| P2C-2 | Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each) | $35.00 | 200 nmol | Provides 45-90 nmol pure. |
| P2C-1 | Primer Pair, (with probe purchase) Cartridge Purified (up to 30 bases each) | $50.00 | 1 µmol | Provides 225-450 nmol pure. |
- Absorption and Emission Spectra for TAMRA Dye
- Spectral Calibration Instructions
- Absorption Spectrum for BHQ®-0 Dye
- Absorption Spectrum for BHQ®-1 Dye
- Absorption Spectrum for BHQ®-2 Dye
- Absorption and Emission Spectra for CAL Fluor® Gold 540 Dye
- Absorption and Emission Spectra for CAL Fluor® Orange 560 Dye
- Absorption and Emission Spectra for CAL Fluor® Red 590 Dye
- Absorption and Emission Spectra for CAL Fluor® Red 610 Dye
- Absorption and Emission Spectra for CAL Fluor® Red 635 Dye
- Absorption and Emission Spectra for FAM Dye
- Absorption and Emission Spectra for Pulsar® 650 Dye
- Absorption and Emission Spectra for Quasar® 570 Dye
- Absorption and Emission Spectra for Quasar® 670 Dye
- Absorption and Emission Spectra for Quasar® 705 Dye
- Absorption and Emission Spectra for TET Dye
- Download MSDS for Unlabeled Oligos
- Download MSDS for Fluorescently-labeled Oligos
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