Other Applications |
BHQ Probes Fuel qPCR – Pick Your Industry
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Dual-Labeled BHQ® Probes are FRET probes for detecting and quantifying specific target sequences during genetic analysis.
Why BHQ Probes?
True dark quencher – no native fluorescence- Broad absorption spectra provides wide quenching range
- Participate in FRET and static quenching for high signal-to-noise ratios and exquisite sensitivity
- Perfect for gene expression analysis, copy number determination, multiplexed qPCR and SNP genotyping
- Selected by the CDC as the integral component for their 2009 H1N1 qPCR test
- Trusted assay component in high-throughput projects such as the NIH Knockout Mouse Project (KOMP). See poster: Oligo Design Across the Mouse Genome for more details
BHQ Probe Characteristics
- Fluorescent-quenched oligo, typically 20-30 bases in length
- Designed to bind at 70 °C for qPCR thermal cycling
- HPLC purified with identity confirmed by ESI-TOF mass spectrometry
- Available in 25 nmol, 50 nmol, 100 nmol, 200 nmol, and 1 umol synthesis scales.
Do you have a large project requiring hundreds of different probes? Biosearch offers custom assay design services to support the molecular testing that is essential to your business. Contact info@biosearchtech.com to discuss how Biosearch can assist in the development of your custom assay.
