Real Time Design
- Design assays online at no charge!
- Express or Custom Modes
- TaqMan® or Amplifluor® Formats
- Optimize Your Assays
Multiplex qPCR Solutions
- Save Time
- Reduce reagent cost
- Duplex, Triplex, 4-plex even 5-plex depending on instrument.
All Major Formats,
More standard dyes.
- An expansive catalog of probe & primer formats means more flexibility in experiment design.
- Wide Range of fluorophores to accomodate all instruments.
No other custom oligo supplier offers you more solutions to help explore the possibilities of qPCR. As a science-driven company with over twenty years of expertise in DNA synthesis, we know what matters most - your time as a researcher. That's why we offer so many ways to help you achieve the best results possible such as RealTimeDesign™ our free online design software that can save real time or our comprehensive line of high-performance, low cost dyes specifically optimized for multiplex qPCR. And Biosearch offers all major probe formats, so no matter what kind of oligo you're designing you know you can get a great price on the highest-quality oligos with great customer service.
Designing optimized, high efficiency probe and primer sets can be an obstacle on the path to exceptional qPCR data. RTD™overcomes this for novice and experienced users alike by incorporating powerful and proven design algorithms in a web-based, on-line software program. Users choose either express or custom modes depending on their level of design expertise. Either design mode generates assays consistently producing amplification efficiencies averaging 99%.
If multiplex qPCR assays are your goal, you next must choose appropriate probe dye & quencher pairs. In singleplex qPCR, the decision is quite simple, FAM paired with BHQ-1, for example. However, in multiplex qPCR the decision is more complex. Biosearch has developed a series of dye & quencher pairs covering the entire visible spectrum based on our CAL Fluor®, Quasar® and BHQ® dyes. We’ve taken the guesswork out of dye selection by providing optimized dye/quencher pairs validated for multiplex qPCR analysis on ALL major real-time thermocyclers.
From viral load quantification to SNP detection assays, your assays may require more than simple TaqMan® probes Biosearch custom synthesizes all major probe/primer formats including: TaqMan®, Molecular Beacon, Amplifluor® Direct, BHQplus™, Scorpion™ and Plexor™ primers.
