By Phone:
USA & Canada:
1.800.GENOME1
International:
1.993.8400
By Fax:
+1.415.883.8488
Or order online 24/7!
Plexor primer technology for real-time, quantitative PCR is a new and novel technique that requires only two primers for sensitive and specific quantitation of amplified DNA. The Plexor Technology is available from Promega Corporation of Madison, Wisconsin, Plexor primer technology takes advantage of the highly specific interaction between two modified nucleotides, isoguanine (iso-dG) and 5'-methylisocytosine (iso-dC) which form a unique base pair when incorporated in double-stranded DNA and pair only with each other.
In Plexor reactions, one PCR primer is synthesized with an iso-dC residue and a fluorescent label at the 5' end. The second PCR primer is unlabeled. Iso-dGTP nucleotides, modified to include a dabcyl quencher, are included in the PCR reaction mix. During the amplification reaction only dabcyl-iso-dGTP can be incorporated at the position complementary to the iso-dC residue. Incorporation of the dabcyl-iso-dGTP in close proximity to the fluorescent label effectively quenches the fluorescent signal.
The simplicity of primer design is a distinct advantage of the Plexor System. The Plexor primer design software is now ready for primer design at the Promega website and is available to design primers for single and multiplex qPCR reactions. The Plexor primer design software selects the appropriate fluorescent labels for single and multiplex PCR or quantitative reverse-transcription PCR (qRT-PCR) based on the optics of many popular real-time instruments.
To begin designing your Plexor primers and to learn more about the Plexor Technology visit the Promega website.
Once you’ve designed your primers, placing an order with Biosearch Technologies is easy and straightforward. The Plexor primer design software will send you an email with the target name, amplicon size and amplicon location used to generate each of the Plexor primers. Following this information the unlabeled primer and the iso-dC-containing primer along with fluorophore compatible with your specific real-time instrument.
Next, any off-the-shelf Plexor Human Control Gene Primer Sets which were selected will be listed.
Because your real-time instrument may need to be calibrated for the appropriate fluorescent dye linked to your Plexor primer, Biosearch Technologies will include the appropriate Calibration Dye upon request at no charge with your first Plexor primer order. If you require additional calibration dyes, these can be ordered directly on the Biosearch website.
Plexor Pricing and Order Placement
To place your order for all Plexor Primers and Plexor Human Control Gene Primers please go to the Ordering Plexor Primers—Step-by-step Instructions page.
If at anytime you need to check on the status of your order, please email info@biosearchtech.com or call 1.800.436.6631 (US/Canada only) or +1.415.883.8400 with your sales order number and a friendly Customer Support representative will be pleased to assist you.
If you’ve previously ordered Plexor primers from Biosearch Technologies and wish to re-order part or all of that order, call our Customer Service telephone number, 1.800.436.6631 (US/Canada) or +1.415.883.8400, advise the Customer Service representative that you wish to re-order an earlier synthesis and provide the Sales Order Number. If you wish to re-order only certain items from the previous synthesis, please be prepared to provide the Lot Number associated with the line items you wish to order.
For technical support, please email techsupport@biosearchtech.com or call 1.800.436.6631.
