Biosearch carries a variety of commonly used dyes as well as proprietary dyes.
Please refer to the following link for a complete listing of various dyes/fluorophores we carry at Biosearch:
http://www.biosearchtech.com/download/brochures/bti_bhq_selectionchart.pdf
The dyes indicated in BOLD font are the dyes that we carry at Biosearch. If you have any questions regarding availability of dyes, please contact our Technical Support department at techsupport@biosearchtech.com

We recommend preparing a 100 µM stock solution of your probe. To prepare a 100 µM stock of reconstituted probe: Multiply the “total nmol” value found on Certificate of Analysis by 10. The resulting number will be the volume of diluent (in microliters) to add to your probe. Once resuspended in the appropriate volume of dilution buffer, the probe will be at a 100 µM stock solution.

Tip: Be sure to make aliquots of working concentration of probe to prevent freeze thaw cycles that may degrade the probe. We recommend freezing probes either at -20 ºC or -80 ºC. Probes and primers can be stored in this state for over one year.

The number of reactions you can run with a given amount of probe or primer is dependent on a few factors. Such as input concentration of the primer or probe and the final yield of the synthesis product, which can be variable. If you plan to use your primer for PCR, 1 nmol of product will provide for approximately 100 PCR reactions (when the primer is used at a 200 to 250 nmol concentration in a 50 µL reaction). As for probes, 1 nmol of product is usually enough to run 100-300 reactions but again, this depends on the concentration of probe you are using in your reaction.

There are a few reference articles and resources for information on designing TaqMan® assays. These articles have general recommendations on oligonucleotide design, cycling parameters and set-up suggestions.

http://dorakmt.tripod.com/genetics/realtime.html

Here’s the link to another helpful resource:
http://www.gene-quantification.info/
This site offers many of great links to articles on Real Time PCR.

Another great resource is the Yahoo! QPCR listserver:
http://groups.yahoo.com/group/qpcrlistserver/

Also, note that we now offer a user-friendly assay software called Real Time Design. It’s available on our website, free of charge. For information please inquiry at support@biosearchtech.com

Probes should be subjected to minimum number of freeze thaw cycles. Therefore, it is recommended that you prepare and store microvials each having sufficient material for a day’s set of experiment and freeze at -20° C or -80° C. As for long term storage we recommend freezing probes at either -20° C or -80° C. Always protect and minimize your probes from light exposure. We recommend storing probes in amber microtubes or wrapping tubes with foil.

Please let us know if you’d like more information, we can provide a information pamphlet that is a great resource for the entire lab! Email support@biosearchtech.com to request information pamphlet or download from the link below.

http://biosearchtech.com/download/brochures/bti_bhq_handling.pdf

The delivery amount for the Valuprobe is 10 nmole, while the standard $150 probe has a minimum delivery of 10 nmole but averages closer to 20 nmole. The other difference is the level of purification. The Valuprobe is purified using Reverse Phase HPLC so that residual fluorescent contamination and other synthesis impurities are removed. The $150 probe is purified by dual HPLC (anion exchange HPLC followed by reverse phase HPLC) which ensures the absolute highest quality in the manufacture of BHQ-quenched dual label probes.
The level of purification is dependent on the researchers preference for purification stringency. It’s recommended to use dual HPLC purified probes for multiplex reactions. For more detailed information on the probe quality comparisons, please contact techsupport@biosearchtech.com

Loading refers to the conjugation ratio, which is the number of hapten groups conjugated to the protein. It is generally accepted that immunization with a lower density hapten-carrier coupled with low doses of antigen preferentially triggers high affinity antibodies. High immunogen doses and high density of hapten on the carrier tend to elicit a higher response since low affinity antibodies are also elicited.

We typically have product information sheets in the format of pdf documents that contain usage information and chemical properties in the support tab of individual product pages. If for any reason you are unable to find what you're looking for on our website, you can also e-mail us at techsupport@biosearchtech.com

At Biosearch, we carry both commonly used dyes such as FAM and TAMRA as well as dyes we have developed , the Quasar® and CAL Fluor® dyes. Because the optics and filters of thermal cyclers vary. We have compiled a chart detailing dye compatibility which can be found here: http://www.biosearchtech.com/hot/multiplexing.asp

You can also download a PDF of our instrument-dye compatibility chart which also includes a dye selection chart.

The synthesis scale and final yield are not the same because there are several steps in the synthesis of probes and primers. These steps include coupling of each base, cleavage of oligonucleotide from solid support and purification steps.
The combination of these steps cause the final yield of the oligonucleotide to be less than the synthesis scale (starting material)

If you have specific questions regarding minimum yields for a particular probe, please contact Technical Support at techsupport@biosearchtech.com