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BiosearchTech: Stellaris FISH mention in Genome Technology's May issue. Check out article "Cause and Consequence": http://t.co/UPaUTWr0
5/8/2012 4:35:30 PM
BiosearchTech: Stellaris FISH mention in Genome Technology's May issue. Check out article "Cause and Consequence": http://t.co/UPaUTWr0
5/8/2012 4:35:30 PM
How do you determine the brightness of a dye? |
The relative brightness of a dye is calculated by multiplying the extinction coefficient by the fluorescence quantum yield. However the relative brightness is also largely determined by the instrument used. Depending on the instruments’ filters, certain dyes may be easier to detect than others, causing different results to be seen depending on the configuration of the real-time PCR machine.
