What is the difference between Black Hole Quenchers (BHQ) and TAMRA? |
There are significant advantages in using Black Hole Quenchers (BHQ) compared to other commonly used quenchers such as TAMRA. One of the main advantages is that BHQs are dark quenchers and will not emit fluorescence thereby decreasing background fluorescence and increasing the signal to noise ratios when compared to corresponding TAMRA probes. Using BHQs also allows more flexibility in terms of dye choices for multiplexing. The BHQ family readily permits single-tube multiplexing due to the increased variety of reporter dyes than can be effectively quenched with little or no cross-talk between reporters. The broader spectral coverage of the BHQ dyes provides the scientist with a larger pool of distinct, spectrally resolved reporter dyes which simplifies the design, implementation and interpretation of multiplexed hybridization probe assays. Visit our Black Hole Quencher dye webpage for more information.
