Can Biosearch probes (and dyes) be used for in-situ hybridization (FISH) studies?

For RNA FISH assays, Biosearch Technologies offers Stellaris FISH probes. We do not currently support DNA FISH applications. Historically, FISH probes were cDNA labeled enzymatically with multiple fluorophores. A single synthetic oligo labeled with a fluorophore is not sufficiently bright for visualization under a microscope and therefore multiple probes are necessary. With Stellaris FISH Probes, multiple probes hybridize in series along the transcript of interest. Each transcript is resolved as a single diffraction limited spot, producing compelling and intuitive images which clearly illustrate the quantity and location of mRNA in situ.

FAQs