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RealTimeDesign Software

Successful Real-time qPCR Assay

New to RealTimeDesign Software?  Download our Quick Start Guide:

Download RTD Quick Start Guide

Learn more about RealTimeDesign software.  Download our informational brochure:

Download RTD Quick Start Guide

RealTimeDesign Promo

RealTimeDesign (RTD) software from Biosearch Technologies is a free, easy to use, yet powerful design application for real-time qPCR.  RTD offers both Express and Custom Modes to accommodate the different levels of control that assays may require.  The software links directly to NCBI to retrieve sequences according to accession number, and to confirm priming specificity.  Whether you're a novice or seasoned expert, RTD will ensure that your probes and primers will demonstrate robust amplification and detection.


With RealTimeDesign software, you're only a few clicks away from:

  • Designing assays for Multiplex qPCR, SNP Genotyping, and Gene Expression
  • Designing Dual-labeled and BHQplus probes and primers and matching optimal primer pairs
  • Designing anywhere from single assays to high-throughput batches
  • Saving a list of your custom designs in your account  "Design Run History"
  • Choosing from Biosearch's wide selection of dyes including our very own Black Hole Quencher® (BHQ®) dye

Once your assays are designed, you can immediately order your probes and primers online without any copying, pasting, or e-mailing.

Take advantage of our introductory offer for first time users of RealTimeDesign software. To start using our RealTimeDesignsoftware, you must be registered for a Biosearch Technologies' web account.

Multiple promotional offers cannot be combined.  Discount applies to the first purchase of any product designed by RealTimeDesign Software.

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