In addition to our range of standard KASP™ genotyping reagents, we provide a specialised KASP formulation specifically for use with the specialist optics and reduced sample volumes used in the Fluidigm genotyping system. A validation service is available for all KASP assays used with the Fluidigm system. Please note: KASP reagents formulated for the Fluidigm system are not interchangable with our standard KASP reagents.

The small size of the individual reaction chambers within the Fluidigm chips leads to the requirement for a high starting concentration of DNA. For human DNA, the required starting concentration is 60 ng / µL; this figure should be proportionally adjusted based on the genome size of the organism of interest. Where DNA concentration is not sufficient, it is imperative to amplify the specific SNP-containing regions from the template DNA prior to performing genotyping on the Fluidigm system. The technique of pre-amplification can be used to amplify the regions of interest by designing primers specific to these regions, and can be multiplexed. The resultant amplicon mixture can then be diluted and used as template for KASP genotyping on the Fluidigm system.

LGC can supply primers for the pre-amplification reaction in conjunction with each KASP Assay ordered. Please specify that you require pre-amplification primers when placing your order.

If you have any queries about running KASP on the Fluidigm system or performing pre-amplification reactions, please do not hesitate to contact our technical support team.

Old KASP for Fluidigm codes New KASP for Fluidigm codes
KBS-1016-027 KASP Fluidigm V4.0 (400µL) 10,000 reactions
(available until February 2017)

KBS-1016-029 - KASP Fluidigm V4.15 (400µL) 10,000 reactions
 

KBS-1016-028 KASP Fluidigm V4.0 (4mL) 100,000 reactions
(available until February 2017)
KBS-1016-030 KASP Fluidigm V4.15 (4mL)
100,000 reactions

Ordering

  1. Please request a quotation or enquire about pricing. Please clearly state that your requirement is for Fluidigm reagents and not standard KASP reagents.
  2. We will email you with the quotation for your assays and mix and include a sequence submission form.
  3. Review your quotation and if you are happy with it, submit a purchase order (PO) to the address provided.
  4. Next, complete and return the sequence submission form with your sequence/s of interest and polymorphism/s marked to tell us which SNPs / InDels you are interested in.
  5. Assay design (primer selection) will then be carried out using our proprietary Kraken™ software suite.
  6. All three target-specific primers for an assay are combined into a single 2D barcoded tube.
  7. Each custom KBD Assay mix is shipped in a 2D barcoded tube, with an additional printed label.

Primers for pre-amplification reactions are also available. Please contact your local sales representation or our customer service team for more information.  

Delivery

The assay aliquots are provided in 2D barcoded tubes. Our Standard service (not laboratory-validated) provides an estimated delivery time of 2-3 weeks. Our standard delivery service (laboratory-validated) will guarantee delivery of the functionally validated KASP assays in 4-6 weeks, whilst the rapid service will guarantee delivery of the functionally validated KASP assays in 2-3 weeks.

Title Description  
KASP manual Full user guide for running KASP in your own laboratory Download
KASP quick start guide Outline protocol for set up of KASP genotyping reactions Download 
How does KASP work? Overview of how KASP genotyping chemistry works and how data is scored at LGC Download
How much DNA should I send for my genotyping project? This document outlines the amount of DNA required for a genotyping project run in our service laboratories Download
Reference list Partial list of species that have been genotyped using KASP Download
Trial kit manual User guide for running the KASP genotyping trial kit Download
Trial kit quick start guide Outline protocol for running the KASP genotyping trial kit Download
Validation kit manual User guide for running the KASP Genotyping validation kit (only sent to customers that are using instruments that we are not familiar with). Download
Running KASP on the ABI 7500 User manual for setting up and running KASP on the ABI 7500 qPCR instrument Download
Running KASP on the ABI 7900 User manual for setting up and running KASP on the ABI 7900 qPCR instrument Download
Running KASP on the ABI StepOne and StepOne Plus User manual for setting up and running KASP on the ABI StepOne and StepOne Plus qPCR instrument Download
Running KASP on the ABI Viia7 User manual for setting up and running KASP on the ABI ABI Viia7 qPCR instrument Download
Running KASP on the BIO-RAD CFX User manual for setting up and running KASP on the BIO-RAD CFX qPCR instrument Download
Running KASP on the Roche LC480 User manual for setting up and running KASP on the Roche LC480 qPCR instrument Download
Running KASP on the illumina EcoRT User manual for setting up and running KASP on the illumina EcoRT qPCR instrument Download
Running KASP on the Agilent Mx3000 / Mx3005P User manual for setting up and running KASP on the Agilent Mx3000 / Mx3005P instrument Download
Running KASP on Roche LC96 Factsheet gives detailed explanation of the issues
running KASP genotyping on an LC96 instrument
Download
KASP thermal cycling conditions A document containing details of all the commonly used thermal cycling protocols for KASP genotyping. Download
KASP thermal cycling 61-55ºC touchdown protocol The standard KASP thermal cycling conditions.   Download
KASP thermal cycling 68-62ºC touchdown protocol An alternative KASP thermal cycling protocol, typically used for assays with high %GC. Download
KASP thermal cycling 2-step 57ºC protocol An alternative KASP thermal cycling protocol, typically used for assays with low %GC. Download
KASP thermal cycling 65-57ºC touchdown protocol The standard KASP thermal cycling conditions for the Fluidigm platform. Download
KASP thermal cycling recycling protocol The thermal cycling conditions for further cycling (‘recycling’) of KASP genotyping reactions. Download
Running KASP on Thermo Scientific Piko Real User manual for setting up and running KASP on the Thermo Scientific PikoReal instrument Download