KASP™-TF Master Mixes are formulated without Triton™ X-100. In addition to our range of standard KASP genotyping reagents, we provide a specialised KASP-TF formulation specifically for use with the specialist optics and reduced sample volumes used in the Fluidigm genotyping system. A validation service is available for all KASP assays used with the Fluidigm system. Please note: KASP-TF reagents formulated for the Fluidigm system are not interchangable with our standard KASP-TF reagents.

The small size of the individual reaction chambers within the Fluidigm chips leads to the requirement for a high starting concentration of DNA. For human DNA, the required starting concentration is 60 ng / µL; this figure should be proportionally adjusted based on the genome size of the organism of interest. Where DNA concentration is not sufficient, it is imperative to amplify the specific SNP-containing regions from the template DNA prior to performing genotyping on the Fluidigm system. The technique of pre-amplification can be used to amplify the regions of interest by designing primers specific to these regions, and can be multiplexed. The resultant amplicon mixture can then be diluted and used as template for KASP genotyping on the Fluidigm system.

LGC can supply primers for the pre-amplification reaction in conjunction with each KASP Assay ordered. Please specify that you require pre-amplification primers when placing your order.

If you have any queries about running KASP on the Fluidigm system or performing pre-amplification reactions, please do not hesitate to contact our technical support team.

Catalogue number Description
KBS-1050-145

KASP-TF for Fluidigm (400 µL)

KBS-1050-146 KASP-TF for Fluidigm (4 mL)

Ordering

Review our KASP Reagents Guidance Notes for step-by-step guide to ordering KASP reagents and delivery information, or request a quote or enquire about pricing, noting that your requirement is for KASP-TF reagents for Fluidigm in the comments section.

 

Title Description  
KASP manual Full user guide for running KASP in your own laboratory Download
KASP quick start guide Outline protocol for set up of KASP genotyping reactions Download 
KASP recycling quick guide KASP genotyping - guide to further cycling of genotyping reactions Download
How does KASP work? Overview of how KASP genotyping chemistry works and how data is scored at LGC Download
How to submit sequences A guide to how sequence information should be submitted to LGC for KASP assay design Download
KASP assay design for highly similar sequences An explanation of how KASP assays can be designed with an anchor, to differentiate between two highly similar sequences Download
How much DNA should I send for my genotyping project? This document outlines the amount of DNA required for a genotyping project run in our service laboratories Download
Reference list Partial list of species that have been genotyped using KASP Download
Trial kit manual User guide for running the KASP genotyping trial kit Download
Trial kit quick start guide Outline protocol for running the KASP genotyping trial kit Download
Validation kit manual User guide for running the KASP Genotyping validation kit (only sent to customers that are using instruments that we are not familiar with). Download
Running KASP on the ABI 7500 User manual for setting up and running KASP on the ABI 7500 qPCR instrument Download
Running KASP on the ABI 7900 User manual for setting up and running KASP on the ABI 7900 qPCR instrument Download
Running KASP on the ABI StepOne and StepOne Plus User manual for setting up and running KASP on the ABI StepOne and StepOne Plus qPCR instrument Download
Running KASP on the ABI Viia7 User manual for setting up and running KASP on the ABI ABI Viia7 qPCR instrument Download
Running KASP on the BIO-RAD CFX User manual for setting up and running KASP on the BIO-RAD CFX qPCR instrument Download
Running KASP on the Roche LC480 User manual for setting up and running KASP on the Roche LC480 qPCR instrument Download
Running KASP on the illumina EcoRT User manual for setting up and running KASP on the illumina EcoRT qPCR instrument Download
Running KASP on the Agilent Mx3000 / Mx3005P User manual for setting up and running KASP on the Agilent Mx3000 / Mx3005P instrument Download
Running KASP on Roche LC96 Factsheet gives detailed explanation of the issues
running KASP genotyping on an LC96 instrument
Download
KASP thermal cycling conditions A document containing details of all the commonly used thermal cycling protocols for KASP genotyping. Download
KASP thermal cycling 61-55ºC touchdown protocol The standard KASP thermal cycling conditions.   Download
KASP thermal cycling 68-62ºC touchdown protocol An alternative KASP thermal cycling protocol, typically used for assays with high %GC. Download
KASP thermal cycling 2-step 57ºC protocol An alternative KASP thermal cycling protocol, typically used for assays with low %GC. Download
KASP thermal cycling 65-57ºC touchdown protocol The standard KASP thermal cycling conditions for the Fluidigm platform. Download
KASP thermal cycling recycling protocol The thermal cycling conditions for further cycling (‘recycling’) of KASP genotyping reactions. Download
Running KASP on Thermo Scientific Piko Real User manual for setting up and running KASP on the Thermo Scientific PikoReal instrument Download