Choose standard KASP by Design (KBD) service for design and delivery of a KASP assay to your SNP or indel of interest.learn more
Custom KASP genotyping assays
Run one of our free-of-charge trial kits on your qPCR instrument before you running your own DNA samples and KASP assays.learn more
Choose premier KASP on Demand (KOD) service for design and delivery of your KASP assay, plus full validation and optimization of your assay by our scientific team.learn more
KASP™ is our proprietary genotyping technology.
The KASP genotyping reaction comprises three components:
- Sample DNA with the sequence of interest
- KASP Assay Mix (containing the target specific primers)
- KASP-TF Master Mix.
The KASP Assay Mix is specific to the SNP or indel to be targeted and consists of two competitive, allele-specific forward primers and one common reverse primer. Each forward primer incorporates an additional tail sequence that corresponds with one of two universal FRET (fluorescent resonance energy transfer) cassettes present in the KASP-TF Master Mix. All custom KASP assays are available with the additional assay validation service.
KASP-TF Master Mix contains the two universal FRET cassettes (FAM and HEX), ROX™ passive reference dye, Taq polymerase, free nucleotides and MgCl2 in an optimised buffer solution.