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Quasar® 705 C6 dT amidite is used for the 5' or internal labeling of synthetic oligonucleotides for a wide array of applications including dual labeled fluorogenic probes for real time PCR. The Quasar 705 moiety is coupled to a thymine for addition to synthetic oligonucleotides. Quasar 705 fluorophore is an indocarbocyanine dye that fluoresces in the deep red region of the visible spectrum and can be effectively quenched by either BHQ®-2 or BHQ-3 dyes. Quasar 705 dye is a direct replacement for Cy™5.5 dye.

Properties:

  • Formula:

    C89H106F6N8O10P2

  • Molecular Weight:

    1623.78

  • Appearance:

    blue green solid

  • Absorption Maximum (Lambda Max):

    690

  • Extinction Coefficient at Lambda max:

    206000

  • Extinction Coefficient at 260 nm:

    24000

  • Fluorescence Maximum:

    705

Product usage:

  • Synthesis conditions:

    Due to the mild cleavage and deprotection conditions, we recommend using fast deprotecting amidites. Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in sealed vial to ensure that all contents are dissolved. For optimal coupling efficiency, we recommend treating the amidite solution with 3-4 Å activated molecular sieves (10% v/v).

  • Dilution:

    100 µmol/mL

  • Deprotection conditions:

    Oligonucleotides labeled with Quasar 705 dT may be deprotected using concentrated NH4OH for 5 hours at 25 °C or for 1 hour at 60 °C. Some degradation may occur when using NH4OH, the use of methylamine should be avoided.

  • Quasar 705 dT is light sensitive material. Prolonged exposure to light may cause photo bleaching. This product is susceptible to oxidation when exposed to air or stored undessicated.
    Image of cleaved and deprotected structure:
    102325c.gif

  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:

    1038.2

Storage and handling:

  • Shipping conditions:

    +2 to +8 °C

  • Storage conditions:

    -20 °C, desiccated

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