5'-DMT-A(PAC)-Suc-CPG; 500 Å (RNA)
5'-DMT-A(PAC)-Suc-RNA CPG is used for the placement of A on the 3' end of oligoribonucleotides. This support has a 2’ acetyl protecting group which is easily cleaved during deprotection. The 500 Å CPG is best suited for the synthesis of RNA sequences up to 50-mers in length. The use of this 5’-DMT protected RNA support is identical to a DNA support with the same protection groups.
- White Powder
- Synthesis conditions:
- If using PAC protected supports or reagents we recommend using phenoxyacetic anhydride or t.butyl phenoacetal acid anhydride(FAST deprotecting capping) as the CAP A reagent. Standard acetic anhydride (CAP A) can cause acetylation of the PAC protected amines during synthesis.
- Cleavage conditions:
- Use concentrated ammonia for 90 minutes at 25 °C.Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.
We do not recommend using AMA to cleave or deprotect sequences that contain uridine. The AMA may degrade the uridine nucleobase.
- Deprotection conditions:
- When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
Storage and handling:
- Storage and handling:
- Storage conditions:
- -15 to -30 °C
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