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5'-DMT-dA(Bz)-Suc-CPG is used for the placement of dA on the 3' end of oligonucleotides. The 500 Å CPG is useful for the synthesis of oligos up to 50-mers in length, especially when larger amounts of product are desired, as it allows for greater nucleoside loading amounts than do supports with larger pore sizes.

Properties:

  • Chemical Name:

    5'-DMT-dA(Bz)-Suc-CPG; 500 Å

  • Appearance:

    White Powder

  • Extinction Coefficient at 260 nm:

    15200

Spectral properties measured in water of cleaved and deprotected nucleoside.

Product usage:

  • Cleavage conditions:

    Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.

  • Deprotection conditions:

    When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.

  • Image of cleaved and deprotected structure:
    100394c.gif

  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:

    313.2

Storage and handling:

  • Shipping conditions:

    Ambient

  • Storage conditions:

    +2 to +8 °C

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