5'-DMT-dA(Bz)-Suc-CPG; 500 Å
5'-DMT-dA(Bz)-Suc-CPG is used for the placement of dA on the 3' end of oligonucleotides. The 500 Å CPG is useful for the synthesis of oligos up to 50-mers in length, especially when larger amounts of product are desired, as it allows for greater nucleoside loading amounts than do supports with larger pore sizes.
- Chemical Name:
- 5'-DMT-dA(Bz)-Suc-CPG; 500 Å
- White Powder
- Extinction Coefficient at 260 nm:
Spectral properties measured in water of cleaved and deprotected nucleoside.
- Cleavage conditions:
- Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.
- Deprotection conditions:
- When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
Image of cleaved and deprotected structure:
Storage and handling:
- Storage and handling:
- Storage conditions:
- +2 to +8 °C
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