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5'-DMT-G(DMF)-Suc-RNA CPG is used for the placement of G on the 3' end of oligoribonucleotides. . This support has a 2’ acetyl protecting group which is easily cleaved during deprotection. The 500 Å CPG is best suited for the synthesis of RNA sequences up to 50-mers in length. The use of this 5’-DMT protected RNA support is identical to a DNA support with the same protection groups.

Properties:

Product usage:

  • Synthesis conditions:

    Follow the instrument manufacturer's protocol when using this product.

  • Cleavage conditions:

    Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites. We do not recommend using AMA to cleave or deprotect sequences that contain uridine. The AMA may degrade the uridine nucleobase.

  • Deprotection conditions:

    When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.

  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:

    345.2

Storage and handling:

  • Shipping conditions:

    Ambient

  • Storage conditions:

    -15 to -30 °C

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