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  2. WA 0812 2782 5310 Kontraktor Pemborong Interior Rumah Minimalis Sederhana Ukuran 800x600 Daerah Ponjong Gunungkidul

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How do waste handling requirements differ between original sbeadex and sbeadex Lightning?

SBXLCK, SBXLCKSTART

Waste handling requirements do not differ between original sbeadex and sbeadex Lightning. Waste amounts and toxicity for laboratory personnel are significantly reduced with sbeadex Lightning.

I tend to use more of some kit components and less of others, resulting in wastage. Is there an option to order custom volumes?

SBXBLK-01

Yes, it is possible to organise custom configurations and dispensing, depending on purchase volume. Contact us to discuss your needs.

Can I shorten the recommended wash buffer steps or leave a step out?

SBXMXPL, SBXMNPK, SXMXPBS, NAP44628, SBXMNPK-01, NAP41605, SXMXPBS-01, SBXMXPL-01
Depending on species and required DNA quality, the wash times in each wash step can be shortened. We recommend optimising the protocol for your specific sample type and application. Often it is sufficient to wash only once with wash buffer PN1 and once with wash buffer PN2 and omit the water wash step.

My AmpliScribe reaction had excellent yields, but when I ran it on a native gel the RNA was much shorter than the original template. What could have gone wrong?

AS3107, MBTOOL-007

Single stranded in vitro transcripts are best separated using denaturing gels1. Denaturing gels allow in vitro transcripts to separate on the basis of their length rather than based on their length + secondary structure. When using native gels, sample migration may be altered by secondary structures in the transcripts.

What is the best way to purify my protein?

COMCEL-008

We and our customers have used immobilised metal ion affinity chromatography (IMAC) and size exclusion chromatography (SEC) to purify proteins of interest.  Lipid IVA is a homogenous molecule comprising a specific molecular mass and charge making standard purification techniques more effective at removing lipid IVA than LPS. However, a general protein purification protocol does not exist, not even when ClearColi strains are being used for protein expression. Just like with any protein expression platform, the purification scheme largely depends on the specific properties of the protein of interest and must be established in each particular case considering the protein´s physico-chemical properties, size, biological activity, binding affinity etc.

Which FailSafe PreMix should I use for my GC-rich template? I don't want to test all of the PreMixes.

PCRAMP-020, FS99060, PCRAMP-018, PCRAMP-017

It is nearly impossible to determine, without experimentation, which FailSafe PCR PreMix will work best with a given template/primer combination. We strongly recommend using all of the PreMixes for optimisation. Without testing the complete set of FailSafe Premixes, you may not be able to determine the optimal PCR conditions for the specific amplicon that you need.

I want to amplify a template that is ~20 kb. Should I use the FailSafe PCR System or the MasterAmp™ Extra-Long PCR Kit?

FS99100, FS99250, FS9901K, PCRAMP-018

The FailSafe PCR System can amplify templates ~20 kb. For templates >10 kb, we recommend using 2.5 units of the FailSafe PCR Enzyme Mix (for templates <10 kb, use 1.25 units) in 50 µL reactions.

In my end-point assay, the NTC signal was the same as my target signal. Why?

The reaction ran too long and the background NTC signal was allowed to catch up to the maximum sample signal, mostly likely due to primer dimerisation or nonspecific primer annealing and extension events.

See: Why does the signal appear early in my no template control (NTC)?

In my endpoint assay, the NTC signal was the same as my target signal. Why?

The reaction ran too long and the background NTC signal was allowed to catch up to the maximum sample signal, mostly likely due to primer dimerisation or non-specific primer annealing and extension events. See: Why does the signal appear early in my no template control (NTC)?

What is the best way to quantify DNA or RNA isolated using the MasterPure Complete Kit?

NAEXPU-001, MB711400

Spectrophotometric methods (e.g., A260) to quantify DNA or RNA, although in common use, may overestimate the concentration. The best method to quantify DNA is by fluorometry using a DNA-specific dye, such as Hoechst 33258 (bisbenzimide), or PicoGreen® dye (Thermo Fisher Scientific). These dyes bind specifically to double-stranded DNA and not to nucleotides, single-stranded DNA, or RNA. To quantify RNA, we recommend using a Qubit™ fluorometer (Thermo Fisher Scientific) or a 2100 BioAnalyzer® instrument (Agilent).