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What lysis buffers can be used with sbeadex Lightning chemistry?

SBXLCK, SBXLCKSTART

There are 6 available lysis buffers for sbeadex Lightning (PN, PVP, BL, H, UR, LI). This chemistry is also compatible with alternative lysis buffers; these can be trialled alongside the sbeadex Lightning core kits.

To ensure optimal results with the sbeadex Lightning chemistry, lysis buffer optimisation can increase yield and purity of the final nucleic acid eluate.

Do you have any recommendations for lysis of livestock samples?

SBXLCK, SBXLCKSTART

Which lysis buffer to use and length of the lysis reaction depends on the sample material and the downstream needs. Recommendations for specific sample types can be accessed on our website. Livestock tissue benefits from an overnight lysis step with 10 μL Protease K solution (20 mg/mL) per 100 μL lysis reaction. If RNA digestion is required, around 1 μL RNase A (20 mg/mL) per mL lysate can be added during lysis. If Protease K digestion is being performed, we recommend sequential digestion, first with RNase and then with Protease K solution. Alternatively, RNase digestion can be performed during the water wash step.

Do you have any recommendations for lysis of plant samples?

SBXLCK, SBXLCKSTART

Which lysis buffer to use and length of the lysis reaction depends on the sample material and the downstream needs. Recommendations for specific sample types can be accessed on our website. Generally, plant material should be lysed for 10 to 60 minutes. For plant material, protease digestion is strongly recommended, using 2 μL Protease K solution (20 mg/mL) per mL lysis buffer. For plant material with a lot of debris, 40 μL debris capture beads per mL lysis buffer can be added. If RNase digestion is required, around 1 μL RNase (20 mg/mL) per mL lysate can be added during lysis. If Protease K digestion is being performed, we recommend sequential digestion, first with RNase and then with Protease K solution. Alternatively, RNase digestion can be performed during the water wash step.

Does Amp-Seq provide data analysis software to generate genotype calls?

AMPSEQ-001
Biosearch Technologies provides an analysis pipeline (the BiosearchCaller software), compatible with the Amp-Seq technology, to produce genotypes from the sequencing data. The software can be accessed via a cloud system and our technical team will support the deployment and initial setup.

Does Amp-Seq One provide data analysis software to generate genotype calls? 

AMPSEQKIT1
Biosearch Technologies provides an analysis pipeline (the BiosearchCaller software), compatible with the Amp-Seq One technology, to produce genotypes from the sequencing data. The software can be accessed via a cloud system and our technical team will support the deployment and initial setup.

Is RapiDxFire Hot Start Taq compatible with hydrolysis probes?

PCRAMP-005

Yes, RapiDxFire Hot Start Taq is compatible with hydrolysis probes such as BHQ®, BHQplus®, and BHQplex™ coprimer probes.