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You searched for "wa 012 2010 510 kontraktor pemborong interior rural minimal sederhana ukuran 800x600 data ponjong gunungkidul"

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Do you have data indicating concordance of Amp-Seq with other technologies?

AMPSEQ-001

Biosearch Technologies have internal data demonstrating concordance of Amp-Seq results with alternative technologies including Flex-Seq. We tested a panel of diverse samples with Flex-Seq and Amp-Seq with a concordance of 98.82%. If you would like more information about this, please contact techsupport@lgcgroup.com.

Does Amp-Seq provide data analysis software to generate genotype calls?

AMPSEQ-001
Biosearch Technologies provides an analysis pipeline (the BiosearchCaller software), compatible with the Amp-Seq technology, to produce genotypes from the sequencing data. The software can be accessed via a cloud system and our technical team will support the deployment and initial setup.

Do you have data indicating concordance of Amp-Seq One with other technologies? 

AMPSEQKIT1

LGC has a large number of internal datasets demonstrating concordance of Amp-Seq One results with Amp-Seq, which in turn has been tested for concordance with alternative technologies including Flex-Seq. We tested a panel of diverse samples with Flex-Seq and Amp-Seq with a concordance of 98.82%. Concordance between Amp-Seq and Amp-Seq One is 98.19%. If you would like more information, please contact techsupport@lgcgroup.com

Does Amp-Seq One provide data analysis software to generate genotype calls? 

AMPSEQKIT1
Biosearch Technologies provides an analysis pipeline (the BiosearchCaller software), compatible with the Amp-Seq One technology, to produce genotypes from the sequencing data. The software can be accessed via a cloud system and our technical team will support the deployment and initial setup.

Are the Safety Data Sheets (SDS) available in other languages?

PCRAMP-105
Yes, please contact techsupport@LGCGroup.com

Are the Safety Data Sheets (SDS) available in other languages?

Yes, please contact techsupport@LGCGroup.com

What reaction volumes can be used with RapiDxFire qPCR 5X Master Mix GF?

PCRAMP-012

RapiDxFire qPCR Master Mix has been validated for use in qPCR applications in 1.6 µL – 25 µL reaction volumes.

What is the minimal DNA concentration needed prior to starting the enzymatic fragmentation reaction?

NXGSEQ-003, NXGSEQ-004, 15300-1, NXGSEQ-001, NXGSEQ-002

As stated above, you can use a minimum of 50 pg of DNA in your enzymatic fragmentation reaction. Since the maximal volume of input DNA is 12.3 µL for the End-repair/A-tailing Reaction, your digested DNA must be at a concentration >4.1 pg/µL.

Won’t the dyes in the primers affect my Illumina sequencing run and/or data quality?

NXGSEQ-003, NXGSEQ-004, 15300-1, NXGSEQ-001, NXGSEQ-002

No. We’ve done extensive testing to demonstrate that the dyes do not affect the sequencing run/instruments or decrease the quality of the data generated. During the final library clean-up and size selection steps, the dyes are removed and therefore, do not make it on to the sequencer. To ensure compatibility in case of incomplete dye removal, we added the full concentration of dye found in the PCR amplification reactions to our final libraries and sequenced those samples on multiple instruments. Even with this artificially high concentration of dye in the denatured library (worst case scenario), we found no effect on the sequencing run/instrument performance or the yield and quality of the final data.

Is the NxSeq HT Dual Indexing Kit (Item. No. 15300-1) compatible with the NxSeq UltraLow DNA Library Prep Kit, 12 reactions (Item. No. 15012-2)?

NXGSEQ-003, NXGSEQ-004, 15300-1, NXGSEQ-001, NXGSEQ-002

Yes. However if combining these two kits, you will be setting up fewer reactions, and you will need to add 2.5 µL aliquots of one 500 Series Primer and one 700 Series Primer to each library amplification reaction. Be extremely careful to avoid cross-contamination of the Indexed Primers when using only a portion of the supplied volume; open only one tube at a time, and ideally change gloves after closing one tube and before opening the next.