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What is the difference between EpiScript™ RNase H- Reverse Transcriptase and Lyo-Ready EpiScript™ Thermostable Reverse Transcriptase?

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Lyo-Ready EpiScript™ Thermostable Reverse Transcriptase (Lyo-Ready EpiScript Thermostable RT) is an MMLV variant engineered to have increased thermostability over Episcript. It is also formulated to be compatible with lyophilization.

How should I extract or purify my RNA samples if I wish to reverse transcribe them using Lyo-Ready EpiScript Thermostable RT?

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Column-based and magnetic bead-based isolation chemistries are both suitable for use with Lyo-Ready EpiScript™ Thermostable Reverse Transcriptase.

What is the optimum temperature for Lyo-Ready EpiScript™ Thermostable Reverse Transcriptase?

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The optimum temperature for Lyo-Ready EpiScript™ Thermostable Reverse Transcriptase is dependent on a range of factors. These include the target sequence and structure, the type of reverse transcriptase (RT) primer that you are using, and whether you are doing 1-step or 2-step RT-qPCR.

If your target sequence has a high %GC content or a high secondary structure, higher temperatures of 50 °C to 60 °C are better. If random primers are being used in the RT reaction, we do not recommend using temperatures above 42 °C as many hexamers and nonamers will not anneal at 50 °C or 55 °C.

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