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You searched for "Reverse Synthesis"

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What is the difference between EpiScript™ RNase H- Reverse Transcriptase and EpiScriptT RT PLUS?

AS3107, MBTOOL-007, PCRAMP-104

EpiScript RT PLUS contains a blocking mechanism that suppresses reverse transcriptase activity at lower temperatures allowing for room temperature reaction setup.

What is the difference between EpiScript™ RNase H- Reverse Transcriptase and Lyo-Ready EpiScript™ Thermostable Reverse Transcriptase?

30260-1
Lyo-Ready EpiScript™ Thermostable Reverse Transcriptase (Lyo-Ready EpiScript Thermostable RT) is an MMLV variant engineered to have increased thermostability over Episcript. It is also formulated to be compatible with lyophilization.

How should I extract or purify my RNA samples if I wish to reverse transcribe them using Lyo-Ready EpiScript Thermostable RT?

30260-1
Column-based and magnetic bead-based isolation chemistries are both suitable for use with Lyo-Ready EpiScript™ Thermostable Reverse Transcriptase.

Which RT enzyme (MMLV, EpiScript RNase H- Reverse Transcriptase, or EpiScript RT PLUS) should I use for pathogen detection, gene expression, or RNA-Seq library preparation, and why?

AS3107, MBTOOL-007, PCRAMP-104

All three reverse transcriptases; standard MMLV, EpiScript RNase H- Reverse Transcriptase and EpiScript RT PLUS can be used for these applications. EpiScript RNase H- Reverse Transcriptase has the advantage of being active up to 55 °C making it more specific than standard MMLV RT enzymes. EpiScript RT PLUS provides the added benefit of allowing room temperature reaction setup.

What is the longest cDNA product that RapiDxFire Thermostable RT can synthesise?

307

RapiDxFire Thermostable RT has successfully synthesised products up to 1kb in 5 minutes.

What is the highest temperature the cDNA synthesis step can be performed?

PCRAMP-021

The highest temperature for cDNA synthesis is partly dependent on TM of the primers used. Other factors include the length of the desired amplicon and if there is potential RNA secondary structure.

Is EpiScript RNase H- Reverse Transcriptase suitable for RNA-seq library preparation?

221, RDXERT300

Yes, EpiScript RNase H- Reverse Transcriptase is compatible with RNA-Seq.

Can I use EpiScript RNase H- Reverse Transcriptase for monitoring gene expression in mammalian cells?

221, RDXERT300

EpiScript RNase H- Reverse Transcriptase is very useful in the monitoring of gene expression and can be used with both probe hydrolysis and gene intercalation methods of detection.

Which RT enzyme, MMLV or EpiScript RNase H- Reverse Transcriptase, should I use for pathogen detection, gene expression, RNA-Seq library preparation for the human health care market and why?

221, RDXERT300

Both standard MMLV and EpiScript RNase H- Reverse Transcriptase can be used for these human health care applications. EpiScript RNase H- Reverse Transcriptase has the advantage of being active up to 55 °C making it more specific than standard MMLV RT enzymes.

What is ‘template-switching’, and does EpiScript RNase H- Reverse Transcriptase have this capability?

221, RDXERT300

Template-switching is the addition of non-template nucleotides to the 3′ end of the transcript, followed by annealing of a primer. This is followed by second strand synthesis by the reverse transcriptase using its innate DNA polymerase activity. EpiScript RNase H- Reverse Transcriptase does have this capability.

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