Choosing a Probe
A one-size fits all approach to designing qPCR and genotyping assays does not account for different
experimental goals and probe sequence requirements such as percent GC content, secondary structure
avoidance, target Tm values and more. Where one probe mechanism renders it challenging to design around a
difficult target sequence, another will overcome these challenges (e.g. maintaining specificity or quenching
efficiency) without compromising results. At LGC Biosearch we supply you with a toolbox of various
chemistries and probe formats so that you can realize your best results with less effort while saving time
and precious sample.
For guidance on mapping probe choice to common qPCR/PCR applications
visit our Probe Application Chart.
How to Order
Design your probe using RealTimeDesign™ Software. Access the software program and download a copy of
our quick start guide here.
Use the spectral overlay tool to choose the right dye and fluorophore combination for your instrument.
Verify that the dyes (fluorophore and quencher) are spectrally distinct enough for the signals not to
bleed through into each other.
Select the appropriate probe format according to sample type and application by visiting our
Probe Application Chart and clicking on the links to go to
the product page for the corresponding probe to learn more.
To order, locate the "Order" button
on the corresponding probe product page and follow the instructions.