Select your dyes

Dyes in BOLDFACE are available from LGC, Biosearch Technologies

Fluorophore ABS EM Quencher

BHQ® Dye FRET Quenching Range

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    Spectral Overlay Chart

    Choosing a Probe

    When designing qPCR and genotyping assays, its critical to select the right probe format and not follow a one-size fits all approach. Meticulous and careful probe design around factors such as GC-content, secondary structure avoidance, target Tm values are necessary to avoid compromising specificity and quenching efficiency. At LGC Biosearch Technologies, we offer various chemistries and probe formats with powerful cutting edge design software all at your fingertips to enable the best results on every run with less effort, less time, less of your precious sample, and greater confidence.

    For guidance on mapping probe choice to common qPCR/PCR applications visit our Probe Application Chart.