qPCR application chart
For guidance on mapping probe choice to common qPCR/PCR applications.
A one-size fits all approach to designing qPCR and genotyping assays does not account for different experimental goals and probe sequence requirements such as percent GC content, secondary structure avoidance, target Tm values and more. Where one probe mechanism renders it challenging to design around a difficult target sequence, another will overcome these challenges (e.g. maintaining specificity or quenching efficiency) without compromising results. At LGC Biosearch Technologies we supply you with a toolbox of various chemistries and probe formats so that you can realise your best results with less effort while saving time and precious sample.
|BHQplus™ Probes||BHQnova™ Probes||BHQ™ Dual-labeled Probes||Minor groove binder (MGB) probes|
|SNP genotyping/allelic discrimination||✔||✔||✔||✔|
|Gene expression analysis||✔||✔||✔||✔||✔|
|Pathogen detection/viral load quantification||✔||✔||✔||✔||✔|
|AT rich templates||✔||✔||✔||✔||✔|
|Copy number variation||✔||✔||✔||✔||✔|
|Polyploid genotyping, plants||✔||✔||✔|
|Long probe sequence||✔|
How to order
- Use the application chart to discover the appropriate probe(s) for your assay. As there may be some degree of overlap, consider piloting several types of probes during assay development.
- Get started with visiting the probe product page to access specifications such as available dye/quencher options, nmols delivered, etc. for the specific probe type of interest.
- To order, locate the "Order" button on the corresponding probe product page and follow the instructions.