Competent cells for cloning and expression
Unlock better results with our high-quality competent cells for cloning and expression. We offer an unbeatable combination of quality, speed and cost-effectiveness.
Focus on your research, not your cells.
Improve your cloning efficiency and library diversity
From everyday cloning work to complex library construction, let our Competent Cells bring high efficiency and unbeatable value into your lab. Explore our menu of chemical and electrocompetent cells by clicking on the application area below to view all options.
General cloning and library construction
| E. cloni® 10G Chemically Competent Cells | Clone, subclone and propagage plasmids |
| TransforMax™ EC100™ Electrocompetent and Chemically Competent E. coli | Clone and subclone with high-efficiency recA, endA strain |
| E. cloni® 10G and 10GF’ Electrocompetent Cells | Construct libraries, perform general cloning, product ssNDA |
| E. cloni® 5-alpha Chemically Competent Cells | Clone, subclone and transform |
Phage display library applications
Phage Display Competent Cells: Create large phage display libraries and speed discovery
LGC Biosearch Technologies offers a line of competent cells optimised for large-scale library construction, allowing generation of libraries containing billions of unique phage particles.
CRISPR, large or difficult fragment cloning
| Endura™ Competent Cells | Maintain unstable clones and propagate plasmids. Ideal for GeCKO CRISPR library transformation |
| TransforMax™ EPI300TM Electrocompetent and Chemically Competent E. coli | Clone large inserts, control copy number of CopyControl vectors |
| CopyCutter™ EPI400TM Electrocompetent and Chemically Competent E. coli | Clone unstable and toxic sequences with high efficiency |
| TransforMax™ EPI300™-T1R Electrocompetent E. coli | Clone large insersts, control copy number of CopyControlTM vectors and avoid T1 and T5 phage contamination |
| BAC-Optimized Replicator™ v2.0 & 10G BAC-Optimized Electrocompetent Cells | Create BAC libraries. Replicator cells control copy number of pSMART® BAC |
Protein expression
| OverExpress™ C41(DE3) and C43(DE3) Competent Cells | Express difficult or even toxic proteins using any E. coli T7 vector; available as chemically competent or electrocompetent cells |
| E.cloni® Express BL21(DE3) Competent Cells: E. coli | For routine protein expression using any E. coli T7 expression vector; available as chemically competent or highest efficiency electrocompetent cells |
| HI-Control™ BL21(DE3) and HI-Control™ 10G Competent Cells | Gain greater control of protein expressin using the Lacl repressor in two popular cell lines |
Low endotoxin cells
| ClearColi® BL21(DE3) Electrocompetent Cells | Eliminate endotoxin at the source with this unique engineered E. coli strain for low-endotoxin protein expression |
Recovery media
| Recovery Medium | Refined formulation ensures the highest possible transformation efficiency and delivers 10-fold higher efficiency than traditional SOC |
| Expression Recovery Medium | Optimized formulation ensures stability and high-efficiency transformation of protein expression clones |
Get exactly what your research needs
Forget one-size-fits all solutions. Our Custom Competent Cell Preparation services are created specifically with your studies in mind. Let us take care of the difficult work by making any E. coli strain you need competent, and offering flexible formatting options tailored to fit whatever specific requirements you may have – for a wide range of applications:
- Library construction – bulk aliquots for phage display or lentiviral libraries (e.g., CRISPR, shRNA)
- High-throughput cloning – 96-well plates for synthetic biology and automated platforms
- Protein expression – your favorite strain, at highest transformation efficiencies
Get custom competent cells without the hassle
- Personalized service from initial discussion through delivery
- Choose from a variety of flexible options to match your specific needs:
- Style: Chemically competent or electrocompetent cells
- Dispense volume: bulk or small aliquots
- Format: 96-well plates or tubes of your choice
- Strains: Lucigen or any BSL1 E. coli strain of choice that meets your application needs
- High transformation efficiencies that match your needs, up to an industry-leading 4×1010 cfu/µg DNA
- Rapid Turnaround Time – Receive your cells in an average of only 2-3 weeks
- ISO 13485 Certified with 20+ years of manufacturing excellence, available for OEM and partnerships.
- Pilot batches are available so you can test your workflow or E. coli strain.
