BHQ-2 DMT Amidite
BHQ®-2 amidite is used for the 5' labeling of fluorogenic probes or to place the quencher internally. This amidite contains a DMT protecting group, which is removed under traditional conditions. The BHQ-2 quencher was designed to have maximal absorption in the 560 to 670 nm range, which provides excellent quenching of fluorophores emitting in this range.
- Chemical Name:
- 4'-(4-Nitro-phenyldiazo)-2'-methoxy-5'-methoxy-azobenzene-4''-(N-2-oxy ethyl (4,4' dimethoxy trityl))-N-ethyl-
- Molecular Weight:
- purple crystal
- Absorption Maximum (Lambda Max):
- Extinction Coefficient at Lambda max:
- Extinction Coefficient at 260 nm:
Spectral properties measured in PCR buffer as 5'-labeled poly(T) oligo.
- Synthesis Conditions:
- Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in the sealed vial to ensure that all contents are dissolved.
- 100 µmol/mL
- Deprotection Conditions:
- Deprotection conditions are dependent upon the type of amidites used for the synthesis of the oligo. If using standard amidites deprotect in concentrated NH4OH for 5 hours at 60 °C. If using fast deprotecting amidites deprotect in concentrated NH4OH for 1 hour at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA or Rhodamine based dyes) we recommend deprotecting the dye labeled oligonucleotide in t-butylamine/H2O (1:3) for 12-16 hours at 60 °C.
- Additional Information:
- If all the solution is not used during the synthesis, it can be stored under argon and its functionality maintained up to 48 hours.
BHQ-2 amidite is susceptible to oxidation when left out in the open or undessicated.
For detailed information on BHQ fragmentation by MALDI-TOF MS, refer to your BHQ Licensee's Manual Appendix V.
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
Image of cleaved and deprotected structure:
Storage and Handling:
- Storage and Handling:
- Storage Conditions:
- -15 to -30 °C
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All products offered for sale by Biosearch Technologies, Inc. (BTI) are sold under the condition that they be used for research purposes only and are prohibited from use in diagnostic or other commercial applications unless explicitly authorized by separate written agreement with BTI.
Black Hole Quencher® (BHQ), CAL Fluor®, Quasar® and Pulsar® dyes (referred to collectively as “BTI Dyes”) are sold to the purchaser only for internal R&D use, and are not to be used for clinical or diagnostic purposes. Neither the BTI Dyes nor the compounds synthesized with them are to be re-packaged or re-sold. Separate licenses to the BTI Dyes may be available for applications other than the aforementioned internal R&D. Please inquire via firstname.lastname@example.org.
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