Product info

Configure custom research-grade oligos for any application, including unlabeled PCR primers, highly modified oligos, or DNA/RNA hybrid sequences, with our extensive selection of nucleic acid derivatives and specialty oligo building blocks.

Key benefits:

  • ISO 9001:2015-certified quality management system
  • 1000+ oligo modifications
  • Proprietary oligo synthesis instrumentation
  • Demonstrated ability to deliver high quality complex oligos

Configure and order custom oligos

Order online: Use the Order now tab to configure and order your oligo(s) using our online tool. Select from one of our three distinct synthesis scales (50 nmol, 200 nmol, 1 μmol).

Excel order form: If you are ordering many oligos or require orders of >1 μmol synthesis scale, consider using the Build Your Oligo excel order form with online upload and email submission options.

Save time. Use the appropriate ordering option for the type of oligo you need.

Use the Order now tab on this page for unlabelled PCR primers and oligos that don’t fall into the categories below. Use the following links to order these types of oligos:

Choose from a diverse menu of oligo options

Configure bespoke oligos specific to your application with access to more than 1,000 modifications. Popular functional groups include:

  • Amine
  • Biotin
  • Fluorophore
  • Linkage
  • Non-standard base
  • Phosphate
  • Quencher
  • Spacer
  • Standard base
  • Thiol

Modifications Fluorophores and quenchers

We offer several purification strategies ranging from Dual HPLC to salt-free. Following our recommendations, select the appropriate option based on the required purity that is optimal for your application.


Tips for configuring your oligo

RNA bases

Indicate the insertion of an RNA base by typing [rA], [rC], [rG[ or [rU] in the Excel order form or by selecting from the Internal Modification drop down menu in the online configurator. If your oligo sequence is entirely RNA, you can convert to RNA by clicking the Convert to RNA button in the online configurator.

Adding internal modifications

  • When using the online oligo configurator, add internal modifications by selecting from the Internal Modifications drop down menu. The internal modification will be inserted at the point of the cursor.
  • When using the Excel order form, internal modifications can be copied from the list provided in the Excel order form.
  • Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please note that an additional T base will be introduced into the sequence when adding such an internal modification.

Building oligos with wobble/degenerate bases

To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc.

Synthesis scale vs. amount delivered

Delivered amounts are lower than synthesis scale. The final yield (amount delivered) of the oligo is determined by the combination of the modifications requested, the complexity of the oligo design and the stringency of purification selected.

Standard synthesis scales

50 nmol (small)
200 nmol (medium)
1 μmol (large)

For synthesis scales larger than 1 μmol, please specify the exact yield delivered in the notes field in the Excel order form.

Order now

Custom Oligo #{{$index + 1}}

Field required.
Catalog #
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This sequence is too short. Click here for help.
Final yield may be reduced for oligos longer than 50 bases.
This oligo contains more than 150 bases. Click here for help.
This oligo contains multiple 'wobbles' bases. If this oligo is intended to function as a PCR primer we advise limiting to no more that 6 oligo species. Please ensure that you intent to incorperate this degeneracy in the oligo and are aware of the impact on oligo function. Contact technical support for more advice (techsupport@biosearchtech.com)
This oligo contains more than 80 RNA bases. Please reduce this sequence length or click here for help.
If you are ordering an LNA please use an excel order form and email to info@biosearchtech.com
Modifications incompatible. If you would like to order an oligo with an Amino and an internal or 3' Methylene Blue the 5' modification must be Amino C6.
Are you trying to build a fluorogenic probe? If so, please use the appropriate order form. Otherwise, this oligonucleotide will need to be quoted for pricing.
Convert To RNA
Oligo scale
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Item price TBD
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Product listing

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Technical Specs

Sequence length 6-150 bases for DNA
6-80 bases for RNA
Inquire for longer oligos
Purification options Salt-free (desalted)
Reverse Phase Cartridge (RPC)
Reverse Phase HPLC (RP HPLC)
Anion Exchange HPLC (AX HPLC)
Dual HPLC (RP + AX HPLC)
Synthesis scales Standard synthesis scales
  • 50 nmol (small)
  • 200 nmol (medium)
  • 1 μmol (large)

Delivered amounts are lower than synthesis scale. The final yield (amount delivered) of the oligo is determined by the combination of the modifications requested, the complexity of the oligo design and the stringency of purification selected.

Synthesis scales larger than 1 μmol
For synthesis scales larger than 1 μmol, please specify the exact yield delivered in the notes field in the Excel order form.

Modifications and bases Large selection of 5’, 3’ and internal modifications available
Bases include locked nucleic acid (LNA), 2'O-Me RNA and 2'O-MOE RNA
Quality control Mass spectrometry and UHPLC to verify identity and purity
Delivery format Dry or in solution (water, TrisHCl, T10E0.1, T10E1)

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