Custom KASP genotyping assays

Order a KASP assay for your SNP or indel of interest, or choose from one or more of our pre-validated KASP genotyping libraries.

KASP overview

More information about the patented PCR-based KASP™ genotyping advantages are tremendous flexibility, breakthrough cost savings, superb accuracy and performance.

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KASP-TF Master Mix

Order KASP-TF Master Mix independently or together with your KASP Assay. Purchase the optimal version KASP-TF Master Mix for your qPCR instrument.

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KASP™ is our proprietary genotyping technology.

The KASP genotyping reaction comprises three components:

KASP-TF Master Mix and KASP Assay Mix are used in-house for our genotyping service projects but can also be purchased to run KASP genotyping reactions in your own laboratory.

KASP technology


The KASP Assay Mix is specific to the SNP or indel to be targeted and consists of two competitive, allele-specific forward primers and one common reverse primer. Each forward primer incorporates an additional tail sequence that corresponds with one of two universal FRET (fluorescent resonance energy transfer) cassettes present in the KASP-TF Master Mix. All custom KASP assays are available with the additional assay validation service.

KASP-TF Master Mix contains the two universal FRET cassettes (FAM and HEX), ROX™ passive reference dye, Taq polymerase, free nucleotides and MgCl2 in an optimised buffer solution.