Improve assay sensitivity and specificity with locked nucleic acid (LNA) oligonucleotides. LNAs are modified RNA bases in which the ribose is “locked” with a methylene bridge, connecting the 2' oxygen atom to the 4' carbon atom, fixing it in the C3'-endo conformation.
learn moreMinor groove binder (MGB) probes are dual-labelled 5' hydrolysis probes consisting of a 5' fluorescent reporter dye and a 3' Eclipse Dark Quencher (EDQ) conjugated to a MGB moiety. Biosearch Technologies offers high quality yet cost effective MGB oligos with a competitive turnaround time and no minimum order quantity.
learn moreView the selection of fluorophore-quencher combinations for dual-labeled probes available from LGC, Biosearch Technologies. We synthesise high quality dual-labeled probes followed by HPLC purification to ensure every order meets purity requirements. Every oligo receives rigorous QC by electrospray mass spectroscopy before releasing for shipment.
learn moreThe most difficult qPCR applications demand double-quenched probes for optimum performance. BHQnova™ probes have improved quenching efficiency compared to traditional end-labeled probes, while also improving signal release upon amplification. BHQnova is most advantageous in longer probe designs, typically those over 25 bases, to boost the signal-to-noise ratio by overcoming the upper limit on sequence length.
learn moreAn advanced technology for qPCR and SNP genotyping, the BHQplus™ Probe is a compact oligo fortified with our signature duplex stabilising chemistry to elevate the melting temperature and enhance target specificity. BHQplus Probes are the optimal choice for marker-assisted selection in the agricultural industry, and to research the polymorphisms that may influence human health.
learn moreMolecular Beacons have short complementary sequences that fold into a stem-loop structure. This hairpin conformation positions the fluorophore and quencher very close together in space, for remarkably efficient quenching. LGC, Biosearch Technologies currently offers Molecular Beacons labeled with either a Black Hole Quencher™ or a DABCYL dye. These quenchers are paired with a variety of fluorescent reporters.
learn moreScorpions™ Primers combine the probe and primer into one oligo to accomplish a uni-molecular signaling mechanism. Enzymatic hydrolysis of the probe during PCR cycling is not required for signal release. View LGC, Biosearch Technologies’ selection of Scorpions Primers.
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