Custom DNA probes for qPCR

We provide probe and primer formats, including dual-labeled BHQ™ Probes, BHQplus™ Probes, Molecular Beacons and Scorpions™ Primers.

BHQplus Probes

An advanced technology for qPCR and SNP genotyping, the BHQplus™ Probe is a compact oligo fortified with our signature duplex stabilising chemistry to elevate the melting temperature and enhance target specificity. BHQplus Probes are the optimal choice for marker-assisted selection in the agricultural industry, and to research the polymorphisms that may influence human health.

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Locked nucleic acid probes

Improve assay sensitivity and specificity with locked nucleic acid (LNA) oligonucleotides. LNAs are modified RNA bases in which the ribose is “locked” with a methylene bridge, connecting the 2' oxygen atom to the 4' carbon atom, fixing it in the C3'-endo conformation.

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BHQ Dual-Labeled FRET Probes

View the selection of fluorophore-quencher combinations for dual-labeled probes available from LGC, Biosearch Technologies. We synthesise high quality dual-labeled probes followed by HPLC purification to ensure every order meets purity requirements. Every oligo receives rigorous QC by electrospray mass spectroscopy before releasing for shipment.

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BHQnova Probes

The most difficult qPCR applications demand double-quenched probes for optimum performance. BHQnova™ probes have improved quenching efficiency compared to traditional end-labeled probes, while also improving signal release upon amplification. BHQnova is most advantageous in longer probe designs, typically those over 25 bases, to boost the signal-to-noise ratio by overcoming the upper limit on sequence length.

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Molecular Beacons

Molecular Beacons have short complementary sequences that fold into a stem-loop structure. This hairpin conformation positions the fluorophore and quencher very close together in space, for remarkably efficient quenching. LGC, Biosearch Technologies currently offers Molecular Beacons labeled with either a Black Hole Quencher™ or a DABCYL dye. These quenchers are paired with a variety of fluorescent reporters.

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Scorpions Primers

Scorpions™ Primers combine the probe and primer into one oligo to accomplish a uni-molecular signaling mechanism. Enzymatic hydrolysis of the probe during PCR cycling is not required for signal release. View LGC, Biosearch Technologies’ selection of Scorpions Primers.

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