Molecular diagnostic oligos, primers and probes

Quality matters when it comes to molecular diagnostics. Everyone needs to trust in the results, regardless of the lot number, the development phase, and the analytical instruments used. That’s why nine of the largest ten diagnostics companies look to LGC, Biosearch Technologies for their custom oligonucleotides, primers and probes. We deliver quality and reliability across:

Thousands of oligonucleotide modifications, including specialty reagents

Fluorophores and quenchers spanning your full spectrum of needs

Customisable oligos fine-tuned to your specifications

 

Whether you’re ordering research-grade (RUO), GMP-compliant or therapeutic-grade oligos, our sequences are optimally designed, purified and rigorously screened for quality results every time

Dial-up your accuracy

As the inventor of the Black Hole Quencher™ (BHQ™) dyes, Biosearch Technologies has helped set new standards for diagnostic accuracy and reproducibility for more than 35 years.

Selecting the appropriate BHQ labelled probes for your application provides superior quenching, enhanced specificity and optimal signal-to-noise ratios. These attributes make BHQ dyes the quencher of choice for qualitative and quantitative PCR (qPCR) experiments, including single and multiplex gene expression, copy number variation (CNV), single nucleotide polymorphism (SNP) genotyping and presence/absence testing.

 

GMP-grade oligos

GMP-grade oligos are specifically synthesised for in vitro diagnostics (IVD) and molecular diagnostic applications. We meet your regulatory needs from multiple international manufacturing sites, which are ISO 9001:2016 (RUO) and ISO 13485:2016 (CE IVD) certified and 21CFR Parts 820 and 864 compliant, as applicable. Each batch is shipped with a certificate of analysis (CoA), certificate of origin (COO), and more for full QA/QC traceability.

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Dual-labeled BHQ Probes

Dual-labeled BHQ Probes are traditional, linear, FRET probes used on oligos between 20 and 30 bases with a fluorophore and quencher covalently attached to the 5′ and 3′ ends respectively. Choose from a broad spectrum of both proprietary and standard fluorophores and their corresponding Black Hole Quencher dye to obtain high-quality qPCR data.

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BHQplus™ Probes

The BHQplus Probe is a compact probe for qPCR, modified with a duplex-stabilising technology and terminated with BHQ dye. The BHQplus Probe permits the design of shorter oligonucleotides (15 to 25 bases in length) for enhanced specificity and improved mismatch discrimination with modified C and T nucleotides. This probe is useful for detecting more difficult target sequences and genotyping SNPs.

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BHQnova™ Probes

BHQnova Probes offer improved quenching efficiency compared to traditional end-labelled probes and are particularly valuable for longer probe designs (>25 bases in length), as needed for difficult qPCR applications. With the power of both an internal nova quencher and a 3′ BHQ quencher, these longer probes substantially reduce background interference and increase the signal-to-noise ratio.

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Custom oligos (RUO and ISO 9001 certification)

Build your custom DNA, RNA and even DNA/RNA hybrid sequences. Whether you require labelled, unlabelled oligos or PCR primers, our experienced team of scientists are available to help you optimise your design. Most oligos are shipped within seven working days. Certificates of Analysis are available upon request.

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Start with the best

In the early design stages, future operational challenges aren’t often front-of-mind when it comes to scale up for commercial purposes. However, the success of any molecular diagnostics programme plays out over many years. MDx companies must not only design and validate their tests as efficiently as possible, but also get to market quickly with a test that performs consistently throughout its commercial lifetime.

Whether you’re composing a multiplexed assay or pursuing a single target gene, the performance of Biosearch Technologies’ oligos is clear. We deliver that same quality and consistency year after year, as your MDx advances and scales from small nanomoles to millimoles of material.

Quality and consistency are built into our process:

Where other companies scale out—producing many small batches of probes and primers—our production batches increase in size as your needs increase. Larger volumes and fewer batch repetitions keep our oligos performing the same, many years down the road.

We hold ourselves to the highest standards. We’re one of the only manufacturers in the world that can take you from R&D to IVD to therapeutic-grade oligonucleotides. We make our own critical raw components, for ultimate control and continuity throughout our supply chain.

Our oligo synthesis team has a stringent risk mitigation process to safeguard the reliability of your assays. This includes screening for all sequences greater than 45 bases to ensure no template contamination infects the facility.

 

Click here for a behind-the-scenes look at our oligo synthesis process