BHQ-3 DMT Amidite
BHQ®-3 amidite is used for the 5' labeling of fluorogenic probes or to place the quencher internally. This amidite contains a DMT protecting group, which is removed under traditional conditions. The BHQ-3 dye was designed to have maximal absorption in the 620-730 nm range, which provides excellent quenching of fluorophores emitting in this range.
- Chemical Name:
- BHQ-3 DMT Amidite
- Molecular Weight:
- purple solid
- Absorption Maximum (Lambda Max):
- Extinction Coefficient at Lambda max:
- Extinction Coefficient at 260 nm:
Spectral properties measured in PCR buffer as 5'-labeled poly(T) oligo.
- Synthesis Conditions:
- Due to the mild cleavage and deprotection conditions, we recommend using fast deprotecting amidites. Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in sealed vial to ensure that all contents are dissolved. For optimal coupling efficiency, we recommend treating the amidite solution with 3-4 Å activated molecular sieves (10% v/v).
- 100 µmol/mL
- 10-minute coupling
- Deprotection Conditions:
- Oligonucleotides labeled with BHQ-3 amidite may be deprotected using concentrated NH4OH for 45 minutes at 60 °C.
- Additional Information:
- BHQ-3 amidite is susceptible to oxidation when left out in the open or undessicated.
For detailed information on BHQ fragmentation by MALDI-TOF MS, refer to your BHQ Licensee's Manual Appendix V.
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
Image of cleaved and deprotected structure:
Storage and Handling:
- Storage and Handling:
- Storage Conditions:
- -15 to -30 °C
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Permitted Product Usage
All products offered for sale by Biosearch Technologies, Inc. (BTI) are sold under the condition that they be used for research purposes only and are prohibited from use in diagnostic or other commercial applications unless explicitly authorized by separate written agreement with BTI.
Black Hole Quencher® (BHQ), CAL Fluor®, Quasar® and Pulsar® dyes (referred to collectively as “BTI Dyes”) are sold to the purchaser only for internal R&D use, and are not to be used for clinical or diagnostic purposes. Neither the BTI Dyes nor the compounds synthesized with them are to be re-packaged or re-sold. Separate licenses to the BTI Dyes may be available for applications other than the aforementioned internal R&D. Please inquire via firstname.lastname@example.org.
The Black Hole Quencher dye technology is protected in the United States and other countries by U.S. patents and continuations numbered 7,019,129, 7,019,129B1, 7,109,312B2, 7,582,432, 8,410,255B2 and 8,440,399B2 issued to Biosearch Technologies, Inc. The CAL Fluor technology is covered by U.S. patent number 7,344,701B2. The Quasar technology is covered by U.S. Patent numbers 7,705,150B2, 7,868, 157B2 and 8,436,153B2. The Pulsar technology is covered by U.S. Patent numbers 7,635,762B2 and 8,119,781B2.
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