LCG Biosearch Micropure purification columns are intended for reverse-phase purification of 5'-dimethoxytrityl protected oligonucleotides followed by on-column detritylation. The cartridge consists of a column or syringe barrel packed with high performance hydrophobic polymeric resin. The resin allows for strong binding between the 5'-DMT protected product and the resin, thus allowing separation from the most common impurities, truncated sequences, which do not bind. Treatment with acid removes the DMT group from the product. The desired product (free from organic residues) is eluted with 20% acetonitrile. Using the DNA purification columns in conjunction with LGC Biosearch’s Microsync ll vacuum manifold system allows for rapid, efficient purification of many samples simultaneously. Micropure columns may also be used for the simple desalting of oligonucleotides.
|Small and Large Empty DNA Synthesis Columns|
|Microsync Vacuum Manifold System shown with SP-1000 purification cartridges|