2'-O-Methyl-U-Suc-CPG; 500 Å (RNA)
2’-O-Methyl-U-Suc-CPG is used for synthesis of oligonucleotides that contain nuclease resistant 2’-O-methyl linkages to prevent degradation. The 2’-O-Methyl-U-Suc-CPG requires no special handling or deprotection reagents. The 500 Å CPG is best suited for the synthesis of RNA sequences up to 50-mers in length.
- White Powder
- Synthesis conditions:
- Follow the instrument manufacturer's protocol when using this product.
- Cleavage conditions:
- Use concentrated ammonia for 90 minutes at 25°C or for 30 minutes at 60°C.
- Deprotection conditions:
- When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
This RNA support is compatible with deprotection chemistries used with 2’ Fluoro; 2' modified phosphoramidites and TBDMS protected reagents.
- Additional information:
- AMA is not recommended for sequences containing uridine bases.
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
Image of cleaved and deprotected structure:
Storage and handling:
- Storage and handling:
- Storage conditions:
- -15 to -30 °C
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