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BHQ®-1 T-Linker 500Å CPG is used to add the non-fluorescent quencher (BHQ-1) to the 3' end of an oligonucleotide. BHQ-1 quencher has maximal absorption in the 480 to 580 nm range, which provides excellent quenching of fluorophores emitting in this range, such as FAM, TET, JOE, and HEX. The BHQ-1 T-Linker Arm CPG has a 3' phosphate followed by a glycolate linkage to the CPG support, which allows for rapid cleavage of the oligo from the support and is labile enough for base-sensitive oligonucleotides. The 500 Å CPG is useful for the synthesis of oligos up to 50-mers in length, especially when larger amounts of product are desired, as it allows for greater nucleoside loading amounts than do supports with larger pore sizes.

Properties:

  • Appearance:

    dark red powder

  • Absorption Maximum (Lambda Max):

    548

  • Extinction Coefficient at Lambda max:

    41600

  • Extinction Coefficient at 260 nm:

    30100

Spectral properties measured in PCR buffer as 3'-labeled poly(T) oligo.

Product usage:

  • Synthesis conditions:

    Follow the instrument manufacturer's protocol when using this product.

  • Cleavage conditions:

    When using fast deprotecting amidites use concentrated Ammonia for 20 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 5 min at 25 °C; or t-butylamine/H2O (1:3) for 45 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 20 min at 25 °C.

  • Deprotection conditions:

    When using fast deprotecting amidites (eg. C-Ac, G-DMF) deprotect in concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated ammonia for 5 hours at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA) we recommend deprotecting the oligonucleotide in t-butylamine/H2O (1:3) for 6 hours at 60 °C.

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  • The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:

    1098.98

Storage and handling:

  • Shipping conditions:

    Ambient

  • Storage conditions:

    -15 to -30 °C

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