BHQ-1 T Linker Arm, CPG; 500 Å
BHQ®-1 T-Linker 500Å CPG is used to add the non-fluorescent quencher (BHQ-1) to the 3' end of an oligonucleotide. BHQ-1 quencher has maximal absorption in the 480 to 580 nm range, which provides excellent quenching of fluorophores emitting in this range, such as FAM, TET, JOE, and HEX. The BHQ-1 T-Linker Arm CPG has a 3' phosphate followed by a glycolate linkage to the CPG support, which allows for rapid cleavage of the oligo from the support and is labile enough for base-sensitive oligonucleotides. The 500 Å CPG is useful for the synthesis of oligos up to 50-mers in length, especially when larger amounts of product are desired, as it allows for greater nucleoside loading amounts than do supports with larger pore sizes.
- dark red powder
- Absorption Maximum (Lambda Max):
- Extinction Coefficient at Lambda max:
- Extinction Coefficient at 260 nm:
Spectral properties measured in PCR buffer as 3'-labeled poly(T) oligo.
- Synthesis conditions:
- Follow the instrument manufacturer's protocol when using this product.
- Cleavage conditions:
- When using fast deprotecting amidites use concentrated Ammonia for 20 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 5 min at 25 °C; or
t-butylamine/H2O (1:3) for 45 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 20 min at 25 °C.
- Deprotection conditions:
- When using fast deprotecting amidites (eg. C-Ac, G-DMF) deprotect in concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated ammonia for 5 hours at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA) we recommend deprotecting the oligonucleotide in t-butylamine/H2O (1:3) for 6 hours at 60 °C.
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
Storage and handling:
- Storage and handling:
- Storage conditions:
- -15 to -30 °C
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Permitted Product Usage
All products offered for sale by Biosearch Technologies, Inc. (BTI) are sold under the condition that they be used for research purposes only and are prohibited from use in diagnostic or other commercial applications unless explicitly authorized by separate written agreement with BTI.
Black Hole Quencher® (BHQ), CAL Fluor®, Quasar® and Pulsar® dyes (referred to collectively as “BTI Dyes”) are sold to the purchaser only for internal R&D use, and are not to be used for clinical or diagnostic purposes. Neither the BTI Dyes nor the compounds synthesized with them are to be re-packaged or re-sold. Separate licenses to the BTI Dyes may be available for applications other than the aforementioned internal R&D. Please inquire via email@example.com.
The Black Hole Quencher dye technology is protected in the United States and other countries by U.S. patents and continuations numbered 7,019,129, 7,019,129B1, 7,109,312B2, 7,582,432, 8,410,255B2 and 8,440,399B2 issued to Biosearch Technologies, Inc. The CAL Fluor technology is covered by U.S. patent number 7,344,701B2. The Quasar technology is covered by U.S. Patent numbers 7,705,150B2, 7,868, 157B2 and 8,436,153B2. The Pulsar technology is covered by U.S. Patent numbers 7,635,762B2 and 8,119,781B2.
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