Why Real-Time PCR?

Real-time qPCR – Rapid, early, specific and sensitive detection

Viruses and other microbes that cause infection are increasingly identified only through their genetic signatures. Real-time qPCR is the industry standard for DNA analysis, and fluorogenic probes with Black Hole Quencher (BHQ) dyes* are the gold standard for pathogen detection in qPCR. Public health agencies depend upon real-time qPCR for single-molecule sensitivity and impressive dynamic range in their most urgent monitoring programs.

To assist in probe and primer selection, LGC Biosearch offers RealTimeDesign™ (RTD™) software. RealTimeDesign software is a free, web-based assay design application for real-time qPCR that can design probes and primer sets to resolve non-standard pathogens and emerging threats.

*For Research Use Only.  Not for use in diagnostic procedures.

Reliability & Scalability

Reliability & Scalability – From nanomoles to several millimoles

Technical excellence in oligonucleotide synthesis has established LGC Biosearch as the preferred OEM supplier to government laboratories and biodefense contractors. LGC Biosearch’s manufacturing facilities have ample capacity to ramp up production and meet the urgent demands of the medical community, even during a pandemic.

Rapid and precise production is important for robust assays that guard the public health. LGC Biosearch gains manufacturing efficiency through careful control over raw materials: phosphoramidite dyes, quenchers, and solid supports are synthesized in-house as essential precursors for oligo synthesis. LGC Biosearch’s unique synthesizers enable high-throughput oligo synthesis, while automated robotics dispense probes and primers into exacting formulations.

Dual-Labeled BHQ Probes

BHQ Probes & Pathogen Detection

LGC Biosearch is the source of BHQ® Probes. The Black Hole Quencher dyes are renowned for versatile quenching across the visible spectrum, and without native fluorescence of their own. FRET and static quenching mechanisms allow for pairing with a wide range of reporters, including the CAL Fluor® and Quasar® fluorophores invented at LGC Biosearch.

BHQ Probes incorporating these fluorescent reporters achieve excellent signal-to-noise values and expand multiplexed analysis to include reference genes, internal positive controls, or even distinguish a group of organisms. For these reasons, BHQ Probes fuel the microfluidics of field-deployed instruments and are critical reagents in surveillance laboratories that detect and quantify pathogens. In fact, the CDC included BHQ Probes as an integral component in their 2009 H1N1 Panel to detect and identify cases of the novel influenza A (H1N1) virus.

Probes labeled with BHQ dyes have been used to detect or identify the following pathogens in humans:

Avian Flu H5N1
Bordetella pertussis
Burkholderia pseudomallei
Coxiella burnetii
Francisella tularensis (Class Agent)

Hemorrhagic Fever Virus
Hepatitis B/Hepatitis C
Human Immunodeficiency Virus (HIV)
Human Papillomavirus (HPV)
Influenza A/Influenza B
Swine Flu A/H1N1
Vibrio cholerae (cholera)
Yersinia pestis (pneumonia)