SARS-CoV-2 variant identification and detection
Detect the presence and manage the impact of SARS-CoV-2 variants
Worrisome variants of SARS-CoV-2 have challenged health systems to add a new dimension to pandemic preparedness: genomic surveillance. Widespread, standardised efforts to monitor and sequence SARS-CoV-2 samples will enable fast, efficient identification and management of viral mutations.
SARS-CoV-2 is an RNA virus, which tends to have high mutation rates.1 These viral mutations, or variants, may influence viral characteristics such as pathogenicity, transmission rate, host range and more. As such, it is critical that we continue to closely monitor the emergence of these variants as well as track the known, dangerous mutations.
As labs prepare to add genomic surveillance to SARS-CoV-2 diagnostic workflows, it is important to understand the two basic methods to detect and identify known or novel SARS-CoV-2 variants:
Probe-based Reverse Transcription PCR (RT-PCR) genotyping
Confirm the presence of known variants
An RT-PCR-based assay can determine whether that viral sample contains the wild type or variant at that specific, previously identified location in the SARS-CoV-2 genome. This type of assay is comprised of:
Primers: Designed to the flanking regions of the known variation in the RNA genome sequence and used to amplify that target region from cDNA made from each viral RNA sample.
Fluorescently-labelled probe specific for wild type/reference SARS-CoV-2
Differentially-labelled fluorescent probe specific for known variant
Detect your desired variant with SARS-CoV-2 Variant ValuPanels™
Combine the gold-standard quenching power of the BHQ™ quencher with BHQplus™ probes engineered to provide the high-fidelity required for confident genotyping results. Our SARS-CoV-2 Variant ValuPanels consist of separately delivered probes and primers for qualitative detection of specific SARS-CoV-2 mutations by RT-PCR-based genotyping. For Research Use Only. Not for diagnostic procedures.
||Variants containing specified SARS-CoV-2 mutation
|SARS-CoV-2 Variant ValuPanel [E484K]
||B.1.351, B.1.1.28 (P.1)
|SARS-CoV-2 Variant ValuPanel [del H69-V70]
||B.1.1.7, DK mink Cluster V
|SARS-CoV-2 Variant ValuPanel [N501Y]
||B.1.1.7, B.1.351, B.1.1.28 (P.1)
|SARS-CoV-2 Variant ValuPanel [K417N]
|SARS-CoV-2 Variant ValuPanel [K417T]
|SARS-CoV-2 Variant ValuPanel [P681H]
|SARS-CoV-2 Variant ValuPanel [del Y144]
|SARS-CoV-2 Variant ValuPanel [L452R]
Looking to add variant detection to your SARS-CoV-2 testing workflow?
Detect with confidence
Whole genome sequencing (WGS)
Identify known and novel variants to get the full picture
WGS converts the cDNA generated by reverse transcription of a viral RNA genome present in a sample to a sequenceable whole genome SARS-CoV-2 library (e.g. an Illumina-compatible library). In most cases, this method generates overlapping RT-PCR amplicons spanning the entire viral genome, which are converted into NGS library fragments. The key to this approach is to generate overlapping library fragments such that there are no gaps in the final viral genome sequence generated.
Simplify library prep
The NxSeq™ SARS-CoV-2 Whole Genome Library Kit includes primer pairs designed against the Wuhan-Hu-1 strain (NC_045512.2), where the sets are optimised to produce high on-target rates and consistent amplicon yields to maximise multiplexing, streamline analysis and keep costs low. Go from cDNA to final library in just 2 hours!
Examine our rapid library preparation
Drive your sequencing throughput with our service labs
Skip investing in sequencing instrumentation and necessary personnel, and get straight to reliable, actionable data. Our labs serve as an extension of yours when you outsource your WGS project to our global service teams. For Research Use Only. Not for diagnostic procedures.
Explore our service
A side-by-side comparison of the two methods
Probe-based RT-PCR genotyping
Whole genome sequencing
Known and UNKNOWN
New design and testing for each new variant – slower
None required once WGS method is established
Standard, well-accepted method
Greater expertise required
1. Domingo E, Holland JJ. RNA virus mutations and fitness for survival. Annu Rev Microbiol. 1997;51:151-78. doi: 10.1146/annurev.micro.51.1.151. PMID: 9343347.