Technical specifications

For a more detailed review of the quality, robustness and performance of the Amp-Seq method please download the application note or start your custom evaluation.

Amp-Seq is a patent pending, multiplex-PCR based amplicon sequencing technology. The below specifications are applicable to the default Amp‑Seq workflow. Customisation of the workflow is optional.

	Feature	Specifications
Protocol	Default protocol	Fast 2-stage protocol + pooling and clean-up
BioIT	Targeting oligo library design	Available as Biosearch Technologies service, default turnaround time 4-6 weeks
	Optimised oligo library density	Amp-Seq has been validated with up to 5,000 markers
	Data analysis pipeline	BiosearchCaller data analysis pipeline available for on-premises installation and setup
	Variants in flanking sequence	Compatible with de-novo variant discovery
	Typical sequencing depth	Dependent on species and targeting oligo panel
	Marker type compatibility	Dependent on species and targeting oligo panel Single nucleotide polymorphisms (SNPs) and for short (≤10 bp) insertions and deletions (indels), please enquire for options for longer indels and other marker types panel
Reagents	Core reagent system	3 component system, pre-mixing enables just 2 dispensing steps
	Reagent system 'kit' sizes	384 and 3,840 (and multiples of those)
	Reagent system stability	Minimum 1 year shelf life at -20 °C
	Indexing system	Conveniently plated combinatorial dual indexing (CDI) system for up to 9,216 samples per lane
	Bead re-use reagents	Compatible and included by default
DNA	Input DNA quantity	Typically, 5-10 ng (dependent on genome size and primer pool QC)
	HotShot (NaOH) compatibility*	Compatible but not included by default
Equipment	Thermal cycler compatibility	Compatibility data available for Hydrocycler™ and Soellex™, compatibility testing on other platforms through co-development
	Sequencer compatibility	Illumina (other platforms through co-development and customisation)
	Liquid handler compatibility	Biomek i7 compatible, compatibility testing on other platforms optional

* Tested for compatibility on a limited number of species and SNP panels, collaborative pilot testing (see ordering information) will deliver genome and panel specific performance

Key features for the Amp-Seq targeted genotyping by sequencing workflow. Note that the workflow is customisable to your needs.