Biosearch Technologies is part of LGC Diagnostics & Genomics – Learn More
KASP genotyping reagents
Make data-informed breeding decisions faster with our proprietary KASP PCR-based genotyping assay.
Kompetitive Allele Specific PCR (KASP™) assays enable accurate, bi-allelic discrimination of SNPs and indels. Unmatched quality, reliability and credibility have established KASP as a foundational SNP genotyping chemistry for agrigenomics.
- Accurate: KASP has been independently verified to achieve a 99.8% call rate across many DNA extraction techniques, GC/AT-rich templates and plant species.
- Cost effective: KASP does not require expensive labelled assay-specific primers or probes, is compatible with crude DNA extraction methods and uses low reaction volumes.
- Flexible assay design: Our experts will identify the best mix of three non-labelled primers for your SNP, indel or mega indel.
- Open plate reading format: The signal can be read on most FRET-capable plate readers and qPCR instruments. We have downloadable step-by-step protocols for running KASP assays on a wide range of commonly used qPCR instruments.
Access to unparalleled expertise
For more than 21 years, KASP has been the gold standard for PCR-based genotyping. KASP has been used to develop more than 280,000 SNP genotyping assays in more than 75 organisms.
We use KASP in our own labs, giving you access to unmatched expertise and confidence. Whether you need support setting up your KASP genotyping reaction or you’re ready to scale up or diversify your operations, we are here to help.
Contact us about KASP
Getting started with KASP in your lab
Start with an evaluation:
- Request a free trial kit.
- Run the trial kit in your lab with guidance from our technical support team.
- Contact the KASP Assays team to initiate custom primer design.
and validation options
Pre-designed KASP assay panels
In collaboration with scientific partners across the globe, we have developed a number of KASP™ genotyping panels for various species. These pre-designed libraries are cost effective and easy to run with no minimum order quantity.
Unlike chip-based systems with fixed marker sets, our KASP genotyping panels allow you the flexibility to select markers in response to the dynamic nature of marker-assisted breeding projects.
See our menu of pre-designedKASP assay panels
KASP resources
- How to submit sequences
- Sequence submission form
- KASP genotyping protocols for qPCR instruments
- KASP reagents guidance notes to support ordering KASP genotyping assays for your lab
- KASP assay design - anchoring
- End-point genotyping manual compilation
- KASP trial kit manual
- KASP genotyping manual
- DNA requirements for genotyping services
Learn more about applications of KASP genotyping technology.
- Explore the vast applications of KASP genotyping for plant breeding programmes
- Optimise sample collection for aquaculture genotyping
- Increasing resistance to aquaculture disease
- Large-scale genotyping in strawberry genomic selection
- KASP technology accelerates rice improvement in South Asia
- Sustainable pathway for rice growers to meet region demands
Let's talk about KASP
We have been the exclusive supplier of KASP genotyping assays for more than 21 years. Whether you need support setting up your KASP genotyping reaction or you’re ready to scale up or diversify your operations, we are here to help.