Oligo Concentration Calculator
Use an absorption measurement from a spectrophotometer and the extinction coefficient to quickly confirm the concentration of oligos in solution.
IMPORTANT NOTESThe extinction coefficient at 260 nm is found on Biosearch Technologies’ certificate of analysis for each synthesized oligo. Alternately, it can be obtained using the OligoSpec™ Calculator. It is an estimation based upon composite values for each modification and base. The Extinction Coefficient 260 (ε260) is calculated with the formula, ε260 = [(Sum of ε260 for all bases*) + (ε260 for all modifications*)] x 0.9, to adjust for hyperchromicity. All ε260 values are reported in units of M-1•cm-1. ε260 of bases = dA (ε260=15,200), dC(ε260=7,050), dG(ε260=12,010), and T(ε260=8400).
*The extinction coefficient values for all other bases and modifications can be found on BiosearchTech.com under the "Technical Specs" tab available for each oligo modification, or by downloading this PDF.