Now available from LGC Biosearch Technologies: PolyDesign™ proprietary fritted controlled pore glass (CPG)
Meet the growing market demand for high-quality oligos at nanomole or sub-nanomole scales with PolyFrits™.
Traditional particulate solid supports packed into columns were developed for oligo production at scales of 40 nanomoles or higher. PolyDesign technology addresses the shift to small-scale synthesis with low bed-volume frits that embed uniform amounts of solid support particles within an inert porous plastic matrix.
These frits provide a convenient means to handle small quantities of solid support using traditional synthesis methods and techniques.
PolyFrits technology is embedded in the future of high-throughput DNA and RNA oligonucleotide synthesis.
Advantages of PolyFrits
- Uniform distribution of active ingredient with < 10% frit-to-frit variation, often <5%
- Small bed volume, as low as 1 cubic millimetre, to reduce reagent consumption
- High loading capacity, up to 60 vol% active component
- Milder frit manufacturing conditions for better protection of the chemical functional groups on the solid support
How PolyFrit technology contributes to advancing therapeutic applications
Synthetic oligonucleotides are integral to modern molecular biology, facilitating diverse applications from chemical synthesis to the assembly of DNA oligonucleotides into genes, circuits and entire genomes using gene synthesis methods.3
The demand for DNA and RNA oligonucleotide synthesis continues to accelerate because of the increasing role of synthetic oligonucleotide-based therapeutics in healthcare.2 Synthetic RNA oligos, in particular, have garnered clinical interest due to the risks and inherent instability associated with non-modified RNA.1 Therapeutic oligonucleotides derived synthetically are demonstrating efficacy in treating a diverse range of diseases, with hundreds currently in clinical trials and numerous FDA-approved drugs already available.1,4
PolyFrits enable the use of solid support particles in a miniature format for oligonucleotide synthesis. This approach not only provides significant advantages for small-scale synthesis platforms where traditional packed columns are not economically viable but also continues to support the role of synthetic oligonucleotides in therapeutic and clinical applications.
This innovative technology supports a wide array of applications for synthetic oligonucleotides, including:
- Development of antisense gapmer, steric block, splice-switching oligonucleotides, and short interfering RNA drugs5
- Advancement of antagomirs (anti microRNAs), microRNA mimics, aptamers, DNA decoys, and DNAzymes5
- Facilitation of synthetic guide strands for CRISPR/Cas applications5
- Support for innate immunity-stimulating oligonucleotides5
- Exploration of DNA-targeting, triplex-forming oligonucleotides, and strand-invading oligonucleotides5
PolyFrit technology Frequently Asked Questions
Most solid, chemically stable solid supports in powder form under frit manufacturing conditions can be embedded into a frit. The most common supports include controlled pore glass (CPG) and polystyrene. What determines whether or not a support can be incorporated into a frit is the chemical modification on the solid support. Historically, universal CPG has been the most commonly used fritted product in oligonucleotide synthesis, however we are constantly evolving our processes to include more and more of our myriad modified solid supports within PolyFrits.
Customers may also wish to provide us with their own support for incorporation into PolyFrits. Depending on the support type, we can give guidance on suitability and possibilities to meet your needs.
As the world leader in controlled pore glass (CPG) manufacture, we can supply you with several types of universal CPG support for DNA/RNA oligonucleotide synthesis, including the popular UnyLinker™ product.