Sample collection: The all-inclusive service includes a sample collection kit. For plant and aquaculture samples, you will receive a BioArk Leaf, BioArk Seed, or BioArk Fish collection kit. A phytosanitary certificate is not required. The kit stabilizes the sample, resulting in better quality DNA and better genotyping data. Sample collection is not included in KASP genotyping-only projects.

DNA extraction: During project kickoff, we determine the most appropriate extraction chemistry and instrumentation for your sample type and throughput requirements. Once we receive your samples, we extract DNA and conduct quality checks before proceeding to genotyping.

Custom KASP assay design: Our expert scientists develop robust, reproducible KASP assays targeted to your SNPs of interest. New assays are designed using customer-provided SNP sequence information. Your dedicated project manager will assist with assay design. Assays can be reused for repeat projects.

KASP genotyping: Using assay design and DNA template, targeted SNPs are amplified and genotyped. KASP genotyping assays use competitive allele-specific PCR to enable bi-allelic scoring of SNPs and Indels. Learn more about KASP genotyping.

Results report: You’ll receive results in Excel format, viewable using SNPViewer or KlusterCaller software. SNPs or Indels will be sent via email with a secure link to your genotyping data file.

Project conclusion: After completion, project materials are stored for up to 3 months. They will then be disposed of per our standard terms unless you opt for extended storage or material return (additional fees apply).

The standard turnaround for a KASP genotyping service project is four weeks. Projects may be expedited to two weeks with advance arrangement and an additional charge.