KASP primer design and validation



KASP™ has been a go-to choice for genotyping assays by top agriculture breeding programmes worldwide for more than 21 years. KASP is easy to use. Simply add your SNP-specific KASP assay targeting primers (KASP Assay Mix) and the universal KASP-TF Master Mix to your DNA samples then run a thermal cycling program and measure the end-point fluorescence.


If you are new to KASP, start with a free trial kit and get your questions answered by our experts. Once you’ve decided that KASP is a good fit for your application, it’s time to determine how you’d like to leverage our expertise.


  • Primer design: We use our proprietary software to select target-specific primers and send you a custom KASP Assay Mix.
  • Primer design with validation: In addition to primer selection, our experts can fully wet-lab validate and optimise your assay and send you a fully optimised custom KASP Assay Mix.
  • Pre-designed assay libraries: Choose from thousands of pre-validated genotyping assays for several plant and animal species. Learn more.
PCR genotyping
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What is the KASP assay mix?

After you send us your sequence, our in-house experts will use our proprietary software to identify the best mix of three assay-specific non-labelled oligos for your SNP, indel or mega indel:

  • Two allele-specific forward primers
  • One common reverse primer

Aliquots of your KASP Assay Mix are provided in 2D barcoded tubes in sufficient quantities to enable:

  • 2,500 reactions at 10 µL total reaction volumes (96-well PCR plates)
  • 5,000 reactions at 5 µL total reaction volumes (384-well PCR plates)

Start your KASP journey today by requesting a quote for KASP primer design with optional wet-lab services.

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Blend our lab and yours
with our KASP genotyping service.

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KASP resources

Ordering and project set up
Blog posts, webinars and case studies
Data analysis and troubleshooting

Ready to enable KASP in your lab?

We have been the exclusive supplier of KASP genotyping assays for more than 21 years. Use the form below to request a quote for your custom KASP genotyping assay with optional wet lab validation.