Since their introduction to the DNA marketplace in 2000, the Black Hole Quenchers™ have become THE quencher of choice used in qPCR probes and other FRET applications.learn more
Fluorophores and quenchers
Recently issued a patent for its CAL Fluor™ dyes, we introduced this series of vibrant dyes for real-time qPCR as lower-cost, superior performing replacements for many commonly used fluorophores. Their emission maxima range from 544 nm to 637 nm, which make them ideal fluorophores for multiplexed qPCR. They are excellent replacement fluorophores for VIC, TET, HEX, JOE, TAMRA, Texas Red®, LC Red 640® dyes. We offer dye calibration standards to optimise your instruments performance for these dyes.learn more
The Quasar® Dyes were developed as replacements for the Cy™ dyes. These high performing, lower cost dyes facilitate the automated synthesis of probes. With emission values ranging from 566 to 705 nm, Quasar dyes increase fluorophore choices and help eliminate potential cross-talk problems associated with multiplex qPCR and are efficiently quenched by BHQs.learn more
We have developed a fluorescent reporter dye that is expanding the capabilities of both the LightCycler 1.2 and the LightCycler 2.0 real-time, qPCR instruments: Pulsar® 650.learn more
LGC Biosearch Technologies offers CAL Fluor® and Quasar® dyes that span a wide range of emission wavelengths, for maximum flexibility in experimental design. Dye calibration standards are formulated with the fluorophore covalently attached to an oligo-dT (10-mer), to better mimic the signal from a fluorescent probe.learn more
In addition to labeling oligos with FAM, TET, HEX, TAMRA, JOE, ROX, DABCYL, Dabsyl, etc., we have also developed a selection of fluorophores and quenchers with absorption and emission values that span the entire visible spectrum.
LGC, Biosearch Technologies is the proud inventor of the Black Hole Quencher (BHQ) dye! Click here to download our BHQ and Dye Selection Chart.