View tips and guidelines our support team has to offer in real-time PCR, SNP genotyping, gene expression, multiplex qPCR, our proprietary dyes and more.

KASP genotyping

Performing KASP™ genotyping in your own lab is easy. You need your DNA samples, your ordered KASP assays, universal KASP-TF Master Mix from LGC Biosearch Technologies and a FRET-capable plate reader or a qPCR instrument.

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Fluorophores and quenchers

As inventors of the Black Hole Quencher™ (BHQ™) dyes, we design our dyes with the goal of providing solutions for you, the scientist. Our vibrant fluorophores and versatile quenchers are valuable tools for many technologies including: real-time qPCR, multiplex qPCR, SNP genotyping, and FRET assays. See below to learn more about our dyes and the technologies they support.

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DNA Synthesis

In addition to being a custom oligonucleotide manufacturer, LGC Biosearch Technologies is a major supplier of DNA synthesis reagents to oligo synthesis companies around the world.

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Multiplexing qPCR

Multiplexed qPCR combines several PCR assays together into one but resolves the reactions using reporter dyes with distinct spectra. Only Biosearch Technologies offers fluorophores and quenchers that span the spectrum to maximise your multiplexing for 2-plex, 3-plex, 4-plex, and 5-plex qPCR.

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Genotyping - qPCR

Real-time PCR methods using fluorescent probes are ideal for end-point genotyping in low to medium throughput disease-association studies, or marker selection in industrial applications. Review the probe types we offer for this application, and learn how to design assays for genotyping.

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Quenching mechanisms in oligonucleotide probes

The efficiency of fluorescence quenching upon excitation is very distance dependent – if the reporter fluorophore and quencher are far apart, there is fluorescence; if the reporter and quencher are close together in space, fluorescence is suppressed. Continue reading to learn more about static and FRET quenching.

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