Enzymes for IVT mRNA synthesis
mRNA technology jumped into the spotlight of biopharma development following the mass deployment of mRNA-based COVID-19 vaccines. Numerous mRNA therapeutics quickly emerged for a wide range of diseases and are now moving into phase 1 clinical trials.
Developing mRNA-based therapeutics requires rapid, high-quality RNA synthesis. In vitro transcription (IVT) is a method for synthesising RNA that has been instrumental to many of the advancements in RNA-based therapeutics.
The enzymatic generation of mRNA has several steps: plasmid cloning, plasmid linearisation, in vitro transcription,
LGC Biosearch Technologies™ offers high-quality enzymes manufactured in our ISO-13485 accredited manufacturing facilities in Middleton, WI USA with convenient international fulfillment through our global operations.
- Use NxGen T7 RNA Polymerase to catalyse 5′ → 3′ RNA synthesis from the T7 promoter.
- Prevent RNA degradation with RiboGuard™ RNase Inhibitor.
- Digest the DNA template with RNase-Free DNase I.
- Add poly(A) tails to the 3′ ends of IVT RNAs with Poly(A) polymerase tailing kit.
Many of our enzymes are cited in publications as sourced from Lucigen and Epicentre, which are now part of Biosearch Technologies.
Custom projects for IVT RNA synthesis
Backed by 25 years of expertise in enzyme development and production, we also offer custom formulations of in vitro transcription enzymes (IVT enzymes). Like all of our enzymes, we manufacture custom enzymes in our ISO-13485 accredited facilities. We offer customisations to support you from early method development to large-scale production.
Popular enzyme customisations for IVT RNA synthesis:
- Different concentrations
- Triton free or glycerol free
- Custom QC based on your requirements
- Batch reservation
- Lot retention
- Custom storage or reaction buffers
- Bulk dispense
In addition to customising our enzymes to meet your requirements, you can also partner with us to manufacture your proprietary IVT enzyme constructs in our ISO-13485 accredited facilities.
Let's talk enzymes.