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SARS-CoV-2 Variant ValuPanel™ assays consist of separately delivered probes and primers that are designed for qualitative detection of specific SARS-CoV-2 mutations by genotyping using reverse transcription-polymerase chain reaction (RT-PCR). Each SARS-CoV-2 Variant ValuPanel consists of 2 BHQplus™ Probes and 2 primers for amplification and simultaneous discrimination between a specific mutation and the wild-type SARS-CoV-2 sequence.

For Research Use Only. Not for use in diagnostic procedures.

Key benefits

  • Reliable results: From the trusted inventor of Black Hole Quencher™ (BHQ™) technology referenced in 7 of 8 WHO COVID-19 protocols
  • High specificity: Enhanced affinity of the BHQplus Probe technology delivers high-fidelity genotyping results
  • Flexible assay design: Increased assay design options with separately delivered probes and primers 
  • Consistent quality: All probes and primers are HPLC purified and are manufactured and shipped from a facility entirely separate from positive control production.

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ValuPanel SARS-CoV-2 Mutation Variants containing specified SARS-CoV-2 mutation Availability
SARS-CoV-2 Variant ValuPanel [E484K] E484K B.1.351, B.1.1.28 (P.1) Order now
SARS-CoV-2 Variant ValuPanel [del H69-V70] del H69-V70 B.1.1.7, DK mink Cluster V
SARS-CoV-2 Variant ValuPanel [N501Y] N501Y B.1.1.7, B.1.351, B.1.1.28 (P.1)
SARS-CoV-2 Variant ValuPanel [K417N] K417N B.1.351
SARS-CoV-2 Variant ValuPanel [K417T] K417T B.1.1.28 (P.1)
SARS-CoV-2 Variant ValuPanel [P681H] P681H B.1.1.7, B.1.1.238
SARS-CoV-2 Variant ValuPanel [del Y144] del Y144 B.1.1.7 Enquire
SARS-CoV-2 Variant ValuPanel [L452R] L452R B.1.429 (CAL.20C)
       

 

Several variants of SARS-CoV-2 have emerged bringing challenges to diagnostic tests and pandemic eradication efforts. Of particular significance are variants B.1.1.7 (first detected in the United Kingdom), B.1.351 (first detected in South Africa), and P.1 or B.1.1.28 (first identified in travelers from Brazil who arrived in Japan). The ability to quickly and reliably identify SARS-CoV-2 mutations will enhance the ability to gather crucial public health information regarding transmissibility kinetics of new variants, and the efficacy of vaccines and therapeutics.

Product details

SARS-CoV-2 Variant ValuPanel assays consist of separately delivered probes and primers that are designed for qualitative detection of specific SARS-CoV-2 mutations by RT-PCR-based genotyping. Each SARS-CoV-2 Variant ValuPanel consists of 2 BHQplus™ Probes and 2 primers for amplification and simultaneous discrimination between a single mutation and the wild-type SARS-CoV-2 sequence.

 SARS-CoV-2 Variant ValuPanel assays are for research use only, they are not intended for SARS-CoV-2 diagnosis. The assays are intended for secondary, informational tests only, designed for variant screening in respiratory tract samples that have previously tested positive for SARS-CoV-2 by diagnostic RT-PCR. 

Each panel contains the following:

  • Forward and reverse primers that amplify the SARS-CoV-2 target sequence. One primer also initiates reverse transcription of the target sequence.

  • BHQplus™ Probes that specifically detect the mutation site: 

    • A mutation-specific probe labeled with CAL Fluor™ Orange 560

    • A reference/wild type-specific probe labeled with FAM

       

  Oligo type Target Dye Quencher nmol oligo delivered
Panel components Probe Wild-type allele FAM BHQ-1 Plus 5 nmol
Probe Mutant allele CAL Fluor Orange 560 BHQ-1 Plus 5 nmol
Primer N/A N/A N/A 20 nmol
Primer N/A N/A N/A 20 nmol
           



Probes and primers are supplied in dried format, at 5 nmol of each probe and 20 nmol of each primer. Probes and primers are delivered in separate tubes, they are not premixed.

Each kit can perform at least 1000 reactions, based on 20 uL reactions containing 200 nM/probe and 900 nM/primer. Use our Reaction Estimator Tool to calculate the number of reactions achievable when using other reaction volumes and oligo concentrations. 

SARS-CoV-2 Variant ValuPanel assays are designed to be compatible with all qPCR instruments that are capable of detecting FAM and CAL Fluor Orange 560 (VIC/HEX/JOE channel).

Materials not included in the kit:
PCR master mix, ROX reference dye, and positive controls are not included. Positive control template recommendations will be provided for each SARS-CoV-2 Variant ValuPanel.

Analysis of K417T and K417N mutations:
Amino acid position 417 of the Spike protein is an attractive target for genotyping analysis, but as a Multiple Nucleotide Polymorphism (MNP), it may require additional analysis. We recommend that both K417N and K417T assays are used to accurately genotype amino acid 417. Please see the manual for more details.

Resources

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PCR reagents* including master mix and enzyme for SARS-CoV-2 assay development are also available

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sbeadex viral RNA purification kit View product
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For a complete SARS-CoV-2 Quality Solution, consider the AccuPlex™ SARS-CoV-2 Verification Panel coupled with the AccuPlex™ SARS-CoV-2 Reference Material Kit from LGC SeraCare Life Sciences. The target sequence of interest is packaged into a stable non-infectious, and replication-deficient alphavirus particle and the products contain positive materials directed against the published CDC and WHO consensus sequences and the S gene. Also includes negative materials targeting the human RNase P gene.

What do you require to develop your SARS-CoV-2 assay?

We offer probes and primers specific to Coronavirus as well as enzymes, master mix, and extraction capabilities.

Let’s discuss your needs

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*For Research Use Only. Not for use in diagnostic procedures.