Can I use BHQ Probe Master Mix in a 2-step RT-qPCR reaction?

Yes, BHQ Probe Master Mix has been shown to work with high efficiency in 2-step RT-qPCR applications with a range of Biosearch Technologies reverse transciptases, including EpiScript RNase H-Reverse Transcriptase, MMLV High Performance Reverse Transcriptase, NxGen M-MuLV Reverse Transcriptase and RapiDxFire Thermostable Reverse Transcriptase.