How do I know if my detection instrument is compatible? Do I need to recalibrate my instrument?

Biosearch Technologies offers many common fluorophores including FAM, HEX and TAMRA dyes, as well as our own proprietary dyes. Our CAL Fluor® and Quasar® dye series span the spectrum with emission wavelengths ranging from yellow to far-red, and represent alternatives to dyes such as VIC®, Cy™3, Texas Red, LC Red® 640, Cy5, and Cy5.5. For your convenience we have compiled a Multiplexing Dye Recommendations Chart outlining optimal dye combinations in select qPCR machines, as well as a Fluorophore & BHQ® Dye Selection Chart listing reporter-quencher pairings. In addition, you may use our Spectral Overlay Tool to visualize the absorption and emission spectra of multiple dyes together.

Calibration standards are available from Biosearch Technologies to allow for improved signal deconvolution in qPCR instruments and fluorescent plate readers and allows the instrument to store relevant fluorescent profiles of each dye to control for crosstalk between filter channels.

Calibration standards are available for FAM, CAL Fluor and Quasar dyes, and are all available in 5 nmol scales. Please see our qPCR Dye Calibration Standards website for further details.