Are LGC Biosearch Technologies' dyes compatible with DNA sequencing or fragment analyzers?
LGC Biosearch Technologies' dyes have not been tested for compatibility in DNA sequencers or instruments for fragment analysis. While the potential is there, many DNA sequencers are engineered for use with “Big Dye” FRET constructs, such as FAM/NED, to compensate for the limited excitation provided by the laser. LGC Biosearch does not offer these constructs. We encourage users to test our dyes in their instrument prior to designing an assay.
LGC Biosearch dyes are principally intended for use in probe-based studies such as qPCR and SNP genotyping.