What is the difference between BHQ® and BHQplus® Probes?

BHQ® probes are oligonucleotides with a Black Hole Quencher® modification, either internal or at the 3' end. While BHQ probes are typically 20 - 30 bases in length, BHQplus® probes are typically 15 - 25 bases in length. BHQplus probes are an advanced probe type with duplex-stabilizing chemistry to allow for the design of shorter oligonucleotides with relatively high melting temperatures. BHQplus probes are used to detect difficult targets, such as AT-rich regions or SNPs.