What is the difference between the FAM-BHQ ValuProbe™ and the small sized FAM-BHQ probe?
The difference is in the delivery amount and the purification method used. A The difference is in the delivery amount and the purification method used. A ValuProbe™ BHQ® Probe (Cat# DLO-RFB-5) delivers exactly 10 nmol of material, while the small scale FAM-BHQ-1 probe (Cat# DLO-FB1-5) has a minimum delivery of 10 nmol but averages closer to 20 nmol. ValuProbe BHQ Probes are purified using reverse phase HPLC only, whereas the small scale probe is purified by dual-HPLC (anion exchange followed by reverse phase HPLC). Dual-HPLC purification ensures the highest quality of dual-labeled BHQ probes and is recommended for standard probe sequences that will be ordered repeatedly. For more detailed information, please review our Purification Options webpage or contact our Technical Support team.