Why do the sequences for my BHQplus® Probes appear different than before?
With regards to BHQplus® probes, we now fully disclose the modified bases, propynyl-dC (pdC) and propynyl-dU (pdU), responsible for the enhanced binding stability of BHQplus probes. These residues have always been fundamental to the BHQplus chemistry and remain unchanged in composition despite their new presentation. Below is an example of the change in presentation you may expect to appear on the Certificate of Analysis.
Original sequence: ATGCAAGGCATGTGACTGC
New presentation: ApdUGpdCAAGGpdCApdUGpdUGApdCpdUGpdC